Molecular Cloning of Synucleins in River Lamprey Lampetra fluviatilis

O. V. Vorontsova, E. E. Akkuratov, O. M. Korenkova, O. Shupliakov

Research output

Abstract

Synaptic proteins synucleins are found in pathologic aggregates in human brain during neurodegenerative diseases and in some tumors. Normal functions of these proteins in synapses are still unclear. In the present study, we used cDNA cloning to determine amino acid sequences of synucleins in the central nervous system of river lamprey (Lampetra fluviatilis), which is used as a model organism to study molecular mechanisms of synaptic transmission. Three genes are identified. High similarity in amino acid sequences as compared to other vertebrate species is revealed. The bioinformatic analysis predicts that the river lamprey synucleins relate to the group of gamma-synucleins. High homology with human alpha-synuclein is reported. The hydrophobic region required for the formation of alpha-synuclein amyloid fibers is also present in the river lamprey synucleins. The latter suggests that this region appeared at early stages of evolution. The obtained amino acid sequences of synucleins in the river lamprey brain will allow generating novel molecular tools for dissecting physiological functions of these proteins.

Original languageEnglish
Pages (from-to)278-286
Number of pages9
JournalBiochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
Volume12
Issue number3
DOIs
Publication statusPublished - 1 Jul 2018

Fingerprint

Synucleins
Lampreys
Cloning
Molecular Cloning
Rivers
Amino Acid Sequence
Amino Acids
Brain
Neurodegenerative diseases
alpha-Synuclein
Proteins
Neurology
Bioinformatics
Computational Biology
Amyloid
Synaptic Transmission
Neurodegenerative Diseases
Synapses
Vertebrates
Organism Cloning

Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

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abstract = "Synaptic proteins synucleins are found in pathologic aggregates in human brain during neurodegenerative diseases and in some tumors. Normal functions of these proteins in synapses are still unclear. In the present study, we used cDNA cloning to determine amino acid sequences of synucleins in the central nervous system of river lamprey (Lampetra fluviatilis), which is used as a model organism to study molecular mechanisms of synaptic transmission. Three genes are identified. High similarity in amino acid sequences as compared to other vertebrate species is revealed. The bioinformatic analysis predicts that the river lamprey synucleins relate to the group of gamma-synucleins. High homology with human alpha-synuclein is reported. The hydrophobic region required for the formation of alpha-synuclein amyloid fibers is also present in the river lamprey synucleins. The latter suggests that this region appeared at early stages of evolution. The obtained amino acid sequences of synucleins in the river lamprey brain will allow generating novel molecular tools for dissecting physiological functions of these proteins.",
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Molecular Cloning of Synucleins in River Lamprey Lampetra fluviatilis. / Vorontsova, O. V.; Akkuratov, E. E.; Korenkova, O. M.; Shupliakov, O.

In: Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, Vol. 12, No. 3, 01.07.2018, p. 278-286.

Research output

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