Mining seed proteome

From protein dynamics to modification profiles

Andrej Frolov, Tatiana Mamontova, Christian Ihling, Elena Lukasheva, Mikhail Bankin, Veronika Chantseva, Maria Vikhnina, Alena Soboleva, Julia Shumilina, Gregory Mavropolo-Stolyarenko, Tatiana Grischina, Natalia Osmolovskaya, Vladimir Zhukov, Wolfgang Hoehenwarter, Andrea Sinz, Igor Tikhonovich, Ludger A. Wessjohann, Tatiana Bilova, Galina Smolikova, Sergei Medvedev

Research output: Contribution to journalReview articleResearchpeer-review

3 Citations (Scopus)
17 Downloads (Pure)

Abstract

In the modern world, crop plants represent a major source of daily consumed foods. Among them, cereals and legumes — i.e. the crops accumulating oils, carbohydrates and proteins in their seeds — dominate in European agriculture, tremendously impacting global protein consumption and biodiesel production. Therefore, the seeds of crop plants attract the special attention of biologists, biochemists, nutritional physiologists and food chemists. Seed development and germination, as well as age- and stress-related changes in their viability and nutritional properties, can be addressed by a variety of physiological and biochemical methods. In this context, the methods of functional genomics can be applied to address characteristic changes in seed metabolism, which can give access to stress-resistant genotypes. Among these methods, proteomics is one of the most effective tools, allowing mining metabolism changes on the protein level. Here we discuss the main methodological approaches of seed proteomics in the context of physiological changes related to environmental stress and ageing. We provide a comprehensive comparison of gel- and chromatography-based approaches with a special emphasis on advantages and disadvantages of both strategies in characterization of the seed proteome.

Original languageEnglish
Pages (from-to)43-58
Number of pages16
JournalBiological Communications
Volume63
Issue number1
DOIs
StatePublished - 8 Jun 2018

Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Frolov, Andrej ; Mamontova, Tatiana ; Ihling, Christian ; Lukasheva, Elena ; Bankin, Mikhail ; Chantseva, Veronika ; Vikhnina, Maria ; Soboleva, Alena ; Shumilina, Julia ; Mavropolo-Stolyarenko, Gregory ; Grischina, Tatiana ; Osmolovskaya, Natalia ; Zhukov, Vladimir ; Hoehenwarter, Wolfgang ; Sinz, Andrea ; Tikhonovich, Igor ; Wessjohann, Ludger A. ; Bilova, Tatiana ; Smolikova, Galina ; Medvedev, Sergei. / Mining seed proteome : From protein dynamics to modification profiles. In: Biological Communications. 2018 ; Vol. 63, No. 1. pp. 43-58.
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Mining seed proteome : From protein dynamics to modification profiles. / Frolov, Andrej; Mamontova, Tatiana; Ihling, Christian; Lukasheva, Elena; Bankin, Mikhail; Chantseva, Veronika; Vikhnina, Maria; Soboleva, Alena; Shumilina, Julia; Mavropolo-Stolyarenko, Gregory; Grischina, Tatiana; Osmolovskaya, Natalia; Zhukov, Vladimir; Hoehenwarter, Wolfgang; Sinz, Andrea; Tikhonovich, Igor; Wessjohann, Ludger A.; Bilova, Tatiana; Smolikova, Galina; Medvedev, Sergei.

In: Biological Communications, Vol. 63, No. 1, 08.06.2018, p. 43-58.

Research output: Contribution to journalReview articleResearchpeer-review

TY - JOUR

T1 - Mining seed proteome

T2 - From protein dynamics to modification profiles

AU - Frolov, Andrej

AU - Mamontova, Tatiana

AU - Ihling, Christian

AU - Lukasheva, Elena

AU - Bankin, Mikhail

AU - Chantseva, Veronika

AU - Vikhnina, Maria

AU - Soboleva, Alena

AU - Shumilina, Julia

AU - Mavropolo-Stolyarenko, Gregory

AU - Grischina, Tatiana

AU - Osmolovskaya, Natalia

AU - Zhukov, Vladimir

AU - Hoehenwarter, Wolfgang

AU - Sinz, Andrea

AU - Tikhonovich, Igor

AU - Wessjohann, Ludger A.

AU - Bilova, Tatiana

AU - Smolikova, Galina

AU - Medvedev, Sergei

PY - 2018/6/8

Y1 - 2018/6/8

N2 - In the modern world, crop plants represent a major source of daily consumed foods. Among them, cereals and legumes — i.e. the crops accumulating oils, carbohydrates and proteins in their seeds — dominate in European agriculture, tremendously impacting global protein consumption and biodiesel production. Therefore, the seeds of crop plants attract the special attention of biologists, biochemists, nutritional physiologists and food chemists. Seed development and germination, as well as age- and stress-related changes in their viability and nutritional properties, can be addressed by a variety of physiological and biochemical methods. In this context, the methods of functional genomics can be applied to address characteristic changes in seed metabolism, which can give access to stress-resistant genotypes. Among these methods, proteomics is one of the most effective tools, allowing mining metabolism changes on the protein level. Here we discuss the main methodological approaches of seed proteomics in the context of physiological changes related to environmental stress and ageing. We provide a comprehensive comparison of gel- and chromatography-based approaches with a special emphasis on advantages and disadvantages of both strategies in characterization of the seed proteome.

AB - In the modern world, crop plants represent a major source of daily consumed foods. Among them, cereals and legumes — i.e. the crops accumulating oils, carbohydrates and proteins in their seeds — dominate in European agriculture, tremendously impacting global protein consumption and biodiesel production. Therefore, the seeds of crop plants attract the special attention of biologists, biochemists, nutritional physiologists and food chemists. Seed development and germination, as well as age- and stress-related changes in their viability and nutritional properties, can be addressed by a variety of physiological and biochemical methods. In this context, the methods of functional genomics can be applied to address characteristic changes in seed metabolism, which can give access to stress-resistant genotypes. Among these methods, proteomics is one of the most effective tools, allowing mining metabolism changes on the protein level. Here we discuss the main methodological approaches of seed proteomics in the context of physiological changes related to environmental stress and ageing. We provide a comprehensive comparison of gel- and chromatography-based approaches with a special emphasis on advantages and disadvantages of both strategies in characterization of the seed proteome.

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U2 - 10.21638/spbu03.2018.106

DO - 10.21638/spbu03.2018.106

M3 - Review article

VL - 63

SP - 43

EP - 58

JO - Biological Communications

JF - Biological Communications

SN - 2542-2154

IS - 1

ER -