Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization

Natalia Osmolovskaya, Julia Shumilina, Ahyoung Kim, Anna Didio, Tatiana Grishina, Tatiana Bilova, Olga A. Keltsieva, Vladimir Zhukov, Igor Tikhonovich, Elena Tarakhovskaya, Andrej Frolov, Ludger A. Wessjohann

Research output: Contribution to journalReview articlepeer-review

34 Scopus citations
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Abstract

Drought is one of the major stress factors affecting the growth and development of plants. In this context, drought-related losses of crop plant productivity impede sustainable agriculture all over the world. In general, plants respond to water deficits by multiple physiological and metabolic adaptations at the molecular, cellular, and organism levels. To understand the underlying mechanisms of drought tolerance, adequate stress models and arrays of reliable stress markers are required. Therefore, in this review we comprehensively address currently available models of drought stress, based on culturing plants in soil, hydroponically, or in agar culture, and critically discuss advantages and limitations of each design. We also address the methodology of drought stress characterization and discuss it in the context of real experimental approaches. Further, we highlight the trends of methodological developments in drought stress research, i.e., complementing conventional tests with quantification of phytohormones and reactive oxygen species (ROS), measuring antioxidant enzyme activities, and comprehensively profiling transcriptome, proteome, and metabolome.

Original languageEnglish
Article number4089
Number of pages25
JournalInternational Journal of Molecular Sciences
Volume19
Issue number12
Early online date2018
DOIs
StatePublished - 17 Dec 2018

Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Spectroscopy
  • Catalysis
  • Inorganic Chemistry
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Keywords

  • drought stress
  • drought models
  • drought tolerance
  • oxidative stress
  • phytohormones
  • polyethylene glycol (PEG)
  • stress markers
  • GLYCATION END-PRODUCTS
  • HEAT-SHOCK PROTEINS
  • ABIOTIC STRESS
  • ABSCISIC-ACID
  • OSMOTIC-STRESS
  • GENE-EXPRESSION
  • WATER-RELATIONS
  • CHLOROPHYLL FLUORESCENCE
  • POLYETHYLENE GLYCOL-6000
  • OXIDATIVE-DEGRADATION
  • Oxidative stress
  • Drought models
  • Stress markers
  • Drought tolerance
  • Polyethylene glycol (PEG)
  • Drought stress
  • Phytohormones

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