Method of reduction background fluorescence in human fetal brain tissue and quantitative estimate of the effect of photobleaching

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Abstract

We developed the method of reducing the background fluorescence of samples made from formalin-fixed and paraffin-embedded blocks of human fetal brain in the second trimester of gestation. Reduction of excess background fluorescence was carried out in a construction of an original design using an LED lamp with blue and red emission peaks in range of the visible spectrum. The control of the level of decrease in the background autofluorescence was checked by measuring the intensity of the emitted fluorescence of the background of the samples. In addition, we quantitatively measured the relative abundance of immunopositive structures after immunohistochemical staining for control and experimental samples. It has been found that the proposed method reliably reduces the background fluorescence of the samples, which improves the quality of multicolor immunofluorescence images of the cerebral cortex.
Translated title of the contributionМетодика понижения аутофлюоресценции ткани фетального мозга человека и количественная оценка эффекта фотовыцветания
Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalBulletin of Experimental Biology and Medicine
Volume171
Issue number1
DOIs
StatePublished - 1 Jan 2021

Keywords

  • background fluorescence
  • photobleaching
  • FETAL BRAIN INJURY
  • layer-specific markers of neurons

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