Metagenomic Data Assembly – The Way of Decoding Unknown Microorganisms

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14 Scopus citations


Metagenomics is a segment of conventional microbial genomics dedicated to the sequencing and analysis of combined genomic DNA of entire environmental samples. The most critical step of the metagenomic data analysis is the reconstruction of individual genes and genomes of the microorganisms in the communities using metagenomic assemblers – computational programs that put together small fragments of sequenced DNA generated by sequencing instruments. Here, we describe the challenges of metagenomic assembly, a wide spectrum of applications in which metagenomic assemblies were used to better understand the ecology and evolution of microbial ecosystems, and present one of the most efficient microbial assemblers, SPAdes that was upgraded to become applicable for metagenomics.

Original languageEnglish
Article number613791
JournalFrontiers in Microbiology
Early online date23 Mar 2021
StatePublished - 23 Mar 2021

Scopus subject areas

  • Microbiology
  • Microbiology (medical)


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