Metabolomic and physiological changes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) during batch culture development

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10 Scopus citations

Abstract

During mixotrophic batch culturing, the microalga Chlamydomonas reinhardtii goes through S-shaped growth kinetics and cells consistently transit from division to death. Photosynthesis and respiration had two simultaneous maxima at the first half of the exponential growth phase and at the border of the stationary phase. GC-MS analysis detected about 300 compounds of which about 100 were identified. Differences in metabolite profiles were observed between cells sampled at different time points of the growth curve. Several data mining methods clearly indicated that metabolomes tended to group together in a time-dependent manner. Clustering analysis revealed three major groups of metabolites varied in concentration dynamic. The alterations in lipophilic compounds and carbohydrates took place even within the same phase of culture growth, which indicated complex rearrangements in cell biochemistry and physiology. Special attention was paid to alterations in the ratios between metabolite groups. The most significant ratio changes were discovered for pools of amino and fatty acids.
Original languageEnglish
Pages (from-to)803-818
Number of pages16
JournalJournal of Applied Phycology
Volume30
Issue number2
DOIs
StatePublished - Apr 2018

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Aquatic Science
  • Plant Science

Keywords

  • Batch culture
  • Chlamydomonas reinhardtii
  • Metabolomics
  • Multivariate statistics
  • Photosynthesis
  • Respiration

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