Low genetic differentiation between apotheciate Usnea florida and sorediate Usnea subfloridana (Parmeliaceae, Ascomycota) based on microsatellite data

Polina Degtjarenko, Kristiina Mark, Rolands Moisejevs, Dmitry Himelbrant, Irina Stepanchikova, Andrei Tsurykau, Tiina Randlane, Christoph Scheidegger

Research output: Contribution to journalArticlepeer-review

Abstract

Accurate species delimitation has a pivotal role in conservation biology, and it is especially important for threatened species where decisions have political and economic consequences. Finding and applying appropriate character sets and analytical tools to resolve interspecific relationships remains challenging in lichenized fungi. The main aim of our study was to re-assess the species boundaries between Usnea subfloridana and Usnea florida, which have been phylogenetically indistinguishable until now, but are different in reproductive mode and ecological preferences, using fungal-specific simple sequence repeats (SSR), i.e. microsatellite markers. Bayesian clustering analysis, discriminant analysis of principal components (DAPC), minimal spanning network (MSN), and principal component analysis (PCA) failed to separate U. florida and U. subfloridana populations. However, a low significant differentiation between the two taxa was observed across all populations according to AMOVA results. Also, analysis of shared haplotypes and statistical difference in clonal diversity (M) supported the present-day isolation between the apotheciate U. florida and predominantly sorediate U. subfloridana. Our results do not provide a clear support either for the separation of species in this pair or the synonymization of U. florida and U. subfloridana. We suggest that genome-wide data could help resolve the taxonomic question in this species pair.

Original languageEnglish
Pages (from-to)892-902
Number of pages11
JournalFungal Biology
Volume124
Issue number10
DOIs
StatePublished - Oct 2020

Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Keywords

  • Conservation, Speciation process
  • Lichenized fungi
  • Lichens
  • Species boundaries
  • Species pair

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