Looking for Targets to Restore the Contractile Function in Congenital Myopathy Caused by Gln147Pro Tropomyosin

Olga E. Karpicheva, Armen O. Simonyan, Nikita A. Rysev, Charles S. Redwood, Yurii S. Borovikov

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Abstract

We have used the technique of polarized microfluorimetry to obtain new insight into the pathogenesis of skeletal muscle disease caused by the Gln147Pro substitution in β-tropomyosin (Tpm2.2). The spatial rearrangements of actin, myosin and tropomyosin in the single muscle fiber containing reconstituted thin filaments were studied during simulation of several stages of ATP hydrolysis cycle. The angular orientation of the fluorescence probes bound to tropomyosin was found to be changed by the substitution and was characteristic for a shift of tropomyosin strands closer to the inner actin domains. It was observed both in the absence and in the presence of troponin, Ca2+ and myosin heads at all simulated stages of the ATPase cycle. The mutant showed higher flexibility. Moreover, the Gln147Pro substitution disrupted the myosin-induced displacement of tropomyosin over actin. The irregular positioning of the mutant tropomyosin caused premature activation of actin monomers and a tendency to increase the number of myosin cross-bridges in a state of strong binding with actin at low Ca2+.
Original languageEnglish
Number of pages18
JournalInternational Journal of Molecular Sciences
Volume21
Issue number7590
StatePublished - 2020

Scopus subject areas

  • Molecular Biology

Keywords

  • congenital myopathy
  • disease-causing mutations
  • tropomyosin-troponin regulation
  • muscle contraction
  • spatial rearrangements

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