Light scatter by cell suspensions in normal conditions and on exposure to external agents

I. Ya Ostashevskii, A. Yu Sungurov, V. A. Volchkov

Research output: Contribution to journalArticlepeer-review

Abstract

The authors have studied the characteristics of the scatter of light by cell suspensions in norpemal conditions (pH 7·2; 20 °C) and on exposure to external agents (change in pH, rise in temrature). It is shown that in normal conditions for large cells (radius 3-7 μ) the turbidity weakly depends on 1/λ; power n = 0·2-0·3. On damage to the cells the values of n rises and the course of the dependence coincides with the character of the increase in the fraction of damaged cells determined from the eosin test. The changes in the parameters of the total light scatter on damage to the cells are related to the increase in the contribution of scatter by the intracellular microstructure.

Original languageEnglish
Pages (from-to)868-873
Number of pages6
JournalBiophysics
Volume20
Issue number5
StatePublished - 1975

Scopus subject areas

  • Biophysics

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