Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography

Ya A. Dubrovskii, V. D. Gladilovich, I. A. Krasnov, E. P. Podolskaya, E. A. Murashko, V. N. Babakov

Research output

Abstract

Metal-affinity chromatography with Cu2+ containing sorbent was used for separation of globin peptides alkylated by sulfur mustard. It was shown that matrix-assisted laser desorption/ionization time-offlight mass spectrometry allowed isolating peptides alkylated by sulfur mustard (HD) at cysteine-126, -94 and glutamic acid-27 with MH+ of 1444.62, 1561.66, 1676.78 Da, respectively, from rat globin tryptic digest incubated with 60 μM of HD. An alkylated peptide with MH+ of 1444.63 Da was isolated from globin hydrolyzate incubated with 3 μM of HD.

Original languageEnglish
Pages (from-to)41-45
Number of pages5
JournalRussian Journal of Bioorganic Chemistry
Volume38
Issue number1
DOIs
Publication statusPublished - 1 Jan 2012

Fingerprint

Affinity chromatography
Globins
Affinity Chromatography
Mustard Gas
Rats
Hemoglobins
Metals
Peptides
Sorbents
Ionization
Mass spectrometry
Cysteine
Glutamic Acid
Desorption
Mass Spectrometry
Lasers

Scopus subject areas

  • Biochemistry
  • Organic Chemistry

Cite this

Dubrovskii, Ya A. ; Gladilovich, V. D. ; Krasnov, I. A. ; Podolskaya, E. P. ; Murashko, E. A. ; Babakov, V. N. / Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography. In: Russian Journal of Bioorganic Chemistry. 2012 ; Vol. 38, No. 1. pp. 41-45.
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Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography. / Dubrovskii, Ya A.; Gladilovich, V. D.; Krasnov, I. A.; Podolskaya, E. P.; Murashko, E. A.; Babakov, V. N.

In: Russian Journal of Bioorganic Chemistry, Vol. 38, No. 1, 01.01.2012, p. 41-45.

Research output

TY - JOUR

T1 - Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography

AU - Dubrovskii, Ya A.

AU - Gladilovich, V. D.

AU - Krasnov, I. A.

AU - Podolskaya, E. P.

AU - Murashko, E. A.

AU - Babakov, V. N.

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AB - Metal-affinity chromatography with Cu2+ containing sorbent was used for separation of globin peptides alkylated by sulfur mustard. It was shown that matrix-assisted laser desorption/ionization time-offlight mass spectrometry allowed isolating peptides alkylated by sulfur mustard (HD) at cysteine-126, -94 and glutamic acid-27 with MH+ of 1444.62, 1561.66, 1676.78 Da, respectively, from rat globin tryptic digest incubated with 60 μM of HD. An alkylated peptide with MH+ of 1444.63 Da was isolated from globin hydrolyzate incubated with 3 μM of HD.

KW - Alkylation

KW - Hemoglobin

KW - MALDI

KW - Sulfur mustard

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