Involvement of reactive oxygen species in ABA-induced increase in hydraulic conductivity and aquaporin abundance

Guzel Sharipova, Ruslan Ivanov, Dmitriy Veselov, Guzel Akhiyarova, Maria Shishova, Tatyana Nuzhnaya, Guzel Kudoyarova

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The role of reactive oxygen species (ROS) in ABA-induced increase in hydraulic conductivity was hypothesized to be dependent on an increase in aquaporin water channel (AQP) abundance. Single ABA application or its combination with ROS manipulators (ROS scavenger ascorbic acid and NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI)) were studied on detached roots of barley plants. We measured the osmotically driven flow rate of xylem sap and calculated root hydraulic conductivity. In parallel, immunolocalization of ABA and HvPIP2;2 AQPs was performed with corresponding specific antibodies. ABA treatment increased the flow rate of xylem, root hydraulic conductivity and immunostaining for ABA and HvPIP2;2, while the addition of antioxidants prevented the effects of this hormone. The obtained results confirmed the involvement of ROS in ABA effect on hydraulic conductivity, in particular, the importance of H2O2 production by ABA-treated plants for the effect of this hormone on AQP abundance.

Original languageEnglish
Article number9144
JournalInternational Journal of Molecular Sciences
Volume22
Issue number17
DOIs
StatePublished - 1 Sep 2021

Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Keywords

  • ABA
  • Aquaporins
  • Ascorbic acid
  • Diphenyleneiodonium chloride
  • ROS scavenger

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