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Intragenomic Polymorphism of 35S rDNA Internal Transcribed Spacers: Verification of the Hypothesis on the Hybridogenic Origin of Tetraploid Pulsatilla vulgaris. / Punina, E.O.; Gnutikov, A.A.; Nosov, N.N.; Shneyer, V.S.; Rodionov, A.V.

In: Biochemistry (Moscow), Vol. 91, No. 1, 2026, p. 131-144.

Research output: Contribution to journal › Article › peer-review

Harvard

Punina, EO, Gnutikov, AA, Nosov, NN, Shneyer, VS & Rodionov, AV 2026, 'Intragenomic Polymorphism of 35S rDNA Internal Transcribed Spacers: Verification of the Hypothesis on the Hybridogenic Origin of Tetraploid Pulsatilla vulgaris', Biochemistry (Moscow), vol. 91, no. 1, pp. 131-144. https://doi.org/10.1134/s0006297925602722

APA

Punina, E. O., Gnutikov, A. A., Nosov, N. N., Shneyer, V. S., & Rodionov, A. V. (2026). Intragenomic Polymorphism of 35S rDNA Internal Transcribed Spacers: Verification of the Hypothesis on the Hybridogenic Origin of Tetraploid Pulsatilla vulgaris. Biochemistry (Moscow), 91(1), 131-144. https://doi.org/10.1134/s0006297925602722

Vancouver

Punina EO, Gnutikov AA, Nosov NN, Shneyer VS, Rodionov AV. Intragenomic Polymorphism of 35S rDNA Internal Transcribed Spacers: Verification of the Hypothesis on the Hybridogenic Origin of Tetraploid Pulsatilla vulgaris. Biochemistry (Moscow). 2026;91(1):131-144. https://doi.org/10.1134/s0006297925602722

Author

Punina, E.O. ; Gnutikov, A.A. ; Nosov, N.N. ; Shneyer, V.S. ; Rodionov, A.V. / Intragenomic Polymorphism of 35S rDNA Internal Transcribed Spacers: Verification of the Hypothesis on the Hybridogenic Origin of Tetraploid Pulsatilla vulgaris. In: Biochemistry (Moscow). 2026 ; Vol. 91, No. 1. pp. 131-144.

BibTeX

@article{21727c148cda43c88f700ce710e9280b,
title = "Intragenomic Polymorphism of 35S rDNA Internal Transcribed Spacers: Verification of the Hypothesis on the Hybridogenic Origin of Tetraploid Pulsatilla vulgaris",
abstract = "Abstract: Pulsatilla vulgaris (pasque flower) is a rare and endangered plant species. Using ITS1-5.8S rDNA-ITS2 barcode sequencing and locus-specific NGS of the ITS1 spacer, we studied intraspecific and interspecific polymorphism of 35S rDNA loci and investigated the origin of tetraploid P. vulgaris from a unique Russian population. For the first time, the chromosome number in the karyotype of plants from this population was determined as 2n = 32. It was shown that one of the parental species of this tetraploid is the diploid Pulsatilla patens (2n = 16), while the identity of the second parental species remains unknown, although it appears to be closely related to the diploid Pulsatilla pratensis. {\textcopyright} Pleiades Publishing, Ltd. 2026.",
keywords = "35S rRNA genes, allopolyploids, hybrids, ITS1, ITS2, locus-specific NGS (Illumina), plant molecular phylogeny, Pulsatilla, Ranunculaceae, rDNA",
author = "E.O. Punina and A.A. Gnutikov and N.N. Nosov and V.S. Shneyer and A.V. Rodionov",
note = "Export Date: 16 February 2026; Cited By: 0; Correspondence Address: V.S. Shneyer; Komarov Botanical Institute, Russian Academy of Sciences, St. Petersburg, 197022, Russian Federation; email: shneyer@binran.ru; CODEN: BIORA",
year = "2026",
doi = "10.1134/s0006297925602722",
language = "Английский",
volume = "91",
pages = "131--144",
journal = "Biochemistry (Moscow)",
issn = "0006-2979",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "1",

}

RIS

TY - JOUR

T1 - Intragenomic Polymorphism of 35S rDNA Internal Transcribed Spacers: Verification of the Hypothesis on the Hybridogenic Origin of Tetraploid Pulsatilla vulgaris

AU - Punina, E.O.

AU - Gnutikov, A.A.

AU - Nosov, N.N.

AU - Shneyer, V.S.

AU - Rodionov, A.V.

N1 - Export Date: 16 February 2026; Cited By: 0; Correspondence Address: V.S. Shneyer; Komarov Botanical Institute, Russian Academy of Sciences, St. Petersburg, 197022, Russian Federation; email: shneyer@binran.ru; CODEN: BIORA

PY - 2026

Y1 - 2026

N2 - Abstract: Pulsatilla vulgaris (pasque flower) is a rare and endangered plant species. Using ITS1-5.8S rDNA-ITS2 barcode sequencing and locus-specific NGS of the ITS1 spacer, we studied intraspecific and interspecific polymorphism of 35S rDNA loci and investigated the origin of tetraploid P. vulgaris from a unique Russian population. For the first time, the chromosome number in the karyotype of plants from this population was determined as 2n = 32. It was shown that one of the parental species of this tetraploid is the diploid Pulsatilla patens (2n = 16), while the identity of the second parental species remains unknown, although it appears to be closely related to the diploid Pulsatilla pratensis. © Pleiades Publishing, Ltd. 2026.

AB - Abstract: Pulsatilla vulgaris (pasque flower) is a rare and endangered plant species. Using ITS1-5.8S rDNA-ITS2 barcode sequencing and locus-specific NGS of the ITS1 spacer, we studied intraspecific and interspecific polymorphism of 35S rDNA loci and investigated the origin of tetraploid P. vulgaris from a unique Russian population. For the first time, the chromosome number in the karyotype of plants from this population was determined as 2n = 32. It was shown that one of the parental species of this tetraploid is the diploid Pulsatilla patens (2n = 16), while the identity of the second parental species remains unknown, although it appears to be closely related to the diploid Pulsatilla pratensis. © Pleiades Publishing, Ltd. 2026.

KW - 35S rRNA genes

KW - allopolyploids

KW - hybrids

KW - ITS1

KW - ITS2

KW - locus-specific NGS (Illumina)

KW - plant molecular phylogeny

KW - Pulsatilla

KW - Ranunculaceae

KW - rDNA

UR - https://www.mendeley.com/catalogue/e30258b3-9ad7-3a0b-ab10-cdcf2f8bdc3a/

U2 - 10.1134/s0006297925602722

DO - 10.1134/s0006297925602722

M3 - статья

VL - 91

SP - 131

EP - 144

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

SN - 0006-2979

IS - 1

ER -

ID: 148832950