Insulin-mediated downregulation of apolipoprotein A-I gene in human hepatoma cell line HepG2: the role of interaction between FOXO1 and LXRβ transcription factors

V. S. Shavva, A. M. Bogomolova, A. A. Nikitin, E. B. Dizhe, D. A. Tanyanskiy, A. M. Efremov, G. N. Oleinikova, A. P. Perevozchikov, S. V. Orlov

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Apolipoprotein A-I (ApoA-I) is a key component of high density lipoproteins which possess anti-atherosclerotic and anti-inflammatory properties. Insulin is a crucial mediator of the glucose and lipid metabolism that has been implicated in atherosclerotic and inflammatory processes. Important mediators of insulin signaling such as Liver X Receptors (LXRs) and Forkhead Box A2 (FOXA2) are known to regulate apoA-I expression in liver. Forkhead Box O1 (FOXO1) is a well-known target of insulin signaling and a key mediator of oxidative stress response. Low doses of insulin were shown to activate apoA-I expression in human hepatoma HepG2 cells. However, the detailed mechanisms for these processes are still unknown. We studied the possible involvement of FOXO1, FOXA2, LXRα, and LXRβ transcription factors in the insulin-mediated regulation of apoA-I expression. Treatment of HepG2 cells with high doses of insulin (48 h, 100 nM) suppresses apoA-I gene expression. siRNAs against FOXO1, FOXA2, LXRβ, or LXRα abrogated this
Original languageEnglish
JournalJournal of Cellular Biochemistry
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • APOLIPOPROTEIN A-I
  • FOXO1
  • FOXA2
  • LXRα
  • LXRβ
  • INSULIN
  • HepG2

Fingerprint

Dive into the research topics of 'Insulin-mediated downregulation of apolipoprotein A-I gene in human hepatoma cell line HepG2: the role of interaction between FOXO1 and LXRβ transcription factors'. Together they form a unique fingerprint.

Cite this