Influence of the hydroxyapatite particles form on the actine cytoskeleton organization and viability of bone marrow mesenchymal cells

Yu A. Nashchekina, A. S. Chabina, O. M. Osmolovskaya, I. P. Dobrovol’skaya, V. E. Yudin

Research output

Abstract

Hydroxyapatite (HA) is the main inorganic component of bone tissue. To form composite scaffolds with properties identical to native tissue, it is necessary to know how the shape of the HA particles influence on the cell properties. In this study we investigated the effect of the HA particles shape on the actin cytoskeleton organization (fluorescence microscopy) and the viability of bone marrow mesenchymal stromal cells (MTT test). After 5 days of cultivation in the presence of spherical particles, a greater number of viable cells where observed than the number of viable cells when cultured in the presence of needle-like particles. It is suggested that such dependence is due to a higher sorption capacity of proteins by the spherical particles surface having a large specific surface in comparison with the specific surface of needle-shaped particles.

Original languageEnglish
Pages (from-to)813-816
Number of pages4
JournalTsitologiya
Volume60
Issue number10
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Durapatite
Cytoskeleton
Bone Marrow Cells
Mesenchymal Stromal Cells
Needles
Actin Cytoskeleton
Fluorescence Microscopy
Cultured Cells
Cell Count
Bone and Bones
Proteins

Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Nashchekina, Yu A. ; Chabina, A. S. ; Osmolovskaya, O. M. ; Dobrovol’skaya, I. P. ; Yudin, V. E. / Influence of the hydroxyapatite particles form on the actine cytoskeleton organization and viability of bone marrow mesenchymal cells. In: Tsitologiya. 2018 ; Vol. 60, No. 10. pp. 813-816.
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Influence of the hydroxyapatite particles form on the actine cytoskeleton organization and viability of bone marrow mesenchymal cells. / Nashchekina, Yu A.; Chabina, A. S.; Osmolovskaya, O. M.; Dobrovol’skaya, I. P.; Yudin, V. E.

In: Tsitologiya, Vol. 60, No. 10, 01.01.2018, p. 813-816.

Research output

TY - JOUR

T1 - Influence of the hydroxyapatite particles form on the actine cytoskeleton organization and viability of bone marrow mesenchymal cells

AU - Nashchekina, Yu A.

AU - Chabina, A. S.

AU - Osmolovskaya, O. M.

AU - Dobrovol’skaya, I. P.

AU - Yudin, V. E.

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AB - Hydroxyapatite (HA) is the main inorganic component of bone tissue. To form composite scaffolds with properties identical to native tissue, it is necessary to know how the shape of the HA particles influence on the cell properties. In this study we investigated the effect of the HA particles shape on the actin cytoskeleton organization (fluorescence microscopy) and the viability of bone marrow mesenchymal stromal cells (MTT test). After 5 days of cultivation in the presence of spherical particles, a greater number of viable cells where observed than the number of viable cells when cultured in the presence of needle-like particles. It is suggested that such dependence is due to a higher sorption capacity of proteins by the spherical particles surface having a large specific surface in comparison with the specific surface of needle-shaped particles.

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KW - Hydroxyapatite particles

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