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Influence of PDI Gene Overexpression on the Production of Heterologous Proteins in Yeast Pichia pastoris. / Цыганков, Михаил Александрович; Падкина, Марина Владимировна.

In: Russian Journal of Genetics: Applied Research, Vol. 8, No. 2, 01.07.2018, p. 197-205.

Research output: Contribution to journal › Article › peer-review

Harvard

Цыганков, МА & Падкина, МВ 2018, 'Influence of PDI Gene Overexpression on the Production of Heterologous Proteins in Yeast Pichia pastoris', Russian Journal of Genetics: Applied Research, vol. 8, no. 2, pp. 197-205. https://doi.org/10.1134/S2079059718020120

APA

Цыганков, М. А., & Падкина, М. В. (2018). Influence of PDI Gene Overexpression on the Production of Heterologous Proteins in Yeast Pichia pastoris. Russian Journal of Genetics: Applied Research, 8(2), 197-205. https://doi.org/10.1134/S2079059718020120

Vancouver

Цыганков МА, Падкина МВ. Influence of PDI Gene Overexpression on the Production of Heterologous Proteins in Yeast Pichia pastoris. Russian Journal of Genetics: Applied Research. 2018 Jul 1;8(2):197-205. https://doi.org/10.1134/S2079059718020120

Author

Цыганков, Михаил Александрович ; Падкина, Марина Владимировна. / Influence of PDI Gene Overexpression on the Production of Heterologous Proteins in Yeast Pichia pastoris. In: Russian Journal of Genetics: Applied Research. 2018 ; Vol. 8, No. 2. pp. 197-205.

BibTeX

@article{08630b487fc840209d353f9d240f8f05,
title = "Influence of PDI Gene Overexpression on the Production of Heterologous Proteins in Yeast Pichia pastoris",
abstract = "The Pichia pastoris yeast is used for recombinant secretory proteins production. The overexpression of assistant genes, the coding proteins involved in secretion, is one of the approaches used to improve the production of target proteins. PpPDI gene encodes P. pastoris yeast protein disulfide isomerase (Pdi). The aim of our study is to evaluate the effect of Pdi overproduction on the production of recombinant interferons. We obtain the yeast Pichia pastoris strains expressing PpPDI gene under the control of alcohol oxidase-1 (AOX1) promoter. The effect of the overexpression of the PpPDI gene on the production of heterogious proteins—interferon-alfa16 and chicken interferon-gamma—and on the cell growth rate is shown.",
keywords = "Pichia pastoris, heterologous gene expression, Pdi, recombinant interferons, heterologous gene expression, Pdi, Pichia pastoris, recombinant interferons",
author = "Цыганков, {Михаил Александрович} and Падкина, {Марина Владимировна}",
note = "Funding Information: The authors thank A.M. Rumyantsev and A.E. Zob-nina (Laboratory of Biochemical Genetics St. Petersburg State University) for their methodological assistance. The work is supported by grants 1.38.229.2014 and NSH 9513.2016.4 from St. Petersburg State University.",
year = "2018",
month = jul,
day = "1",
doi = "10.1134/S2079059718020120",
language = "English",
volume = "8",
pages = "197--205",
journal = "Russian Journal of Genetics: Applied Research",
issn = "2079-0597",
publisher = "Springer Nature",
number = "2",

}

RIS

TY - JOUR

T1 - Influence of PDI Gene Overexpression on the Production of Heterologous Proteins in Yeast Pichia pastoris

AU - Цыганков, Михаил Александрович

AU - Падкина, Марина Владимировна

N1 - Funding Information: The authors thank A.M. Rumyantsev and A.E. Zob-nina (Laboratory of Biochemical Genetics St. Petersburg State University) for their methodological assistance. The work is supported by grants 1.38.229.2014 and NSH 9513.2016.4 from St. Petersburg State University.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - The Pichia pastoris yeast is used for recombinant secretory proteins production. The overexpression of assistant genes, the coding proteins involved in secretion, is one of the approaches used to improve the production of target proteins. PpPDI gene encodes P. pastoris yeast protein disulfide isomerase (Pdi). The aim of our study is to evaluate the effect of Pdi overproduction on the production of recombinant interferons. We obtain the yeast Pichia pastoris strains expressing PpPDI gene under the control of alcohol oxidase-1 (AOX1) promoter. The effect of the overexpression of the PpPDI gene on the production of heterogious proteins—interferon-alfa16 and chicken interferon-gamma—and on the cell growth rate is shown.

AB - The Pichia pastoris yeast is used for recombinant secretory proteins production. The overexpression of assistant genes, the coding proteins involved in secretion, is one of the approaches used to improve the production of target proteins. PpPDI gene encodes P. pastoris yeast protein disulfide isomerase (Pdi). The aim of our study is to evaluate the effect of Pdi overproduction on the production of recombinant interferons. We obtain the yeast Pichia pastoris strains expressing PpPDI gene under the control of alcohol oxidase-1 (AOX1) promoter. The effect of the overexpression of the PpPDI gene on the production of heterogious proteins—interferon-alfa16 and chicken interferon-gamma—and on the cell growth rate is shown.

KW - Pichia pastoris, heterologous gene expression, Pdi, recombinant interferons

KW - heterologous gene expression

KW - Pdi

KW - Pichia pastoris

KW - recombinant interferons

UR - http://www.scopus.com/inward/record.url?scp=85050025576&partnerID=8YFLogxK

U2 - 10.1134/S2079059718020120

DO - 10.1134/S2079059718020120

M3 - Article

VL - 8

SP - 197

EP - 205

JO - Russian Journal of Genetics: Applied Research

JF - Russian Journal of Genetics: Applied Research

SN - 2079-0597

IS - 2

ER -

ID: 29163414