Indirect monitoring of protein A biosynthesis in E.coli using potentiometric multisensor system

D. Kirsanov, A. Korepanov, D. Dorovenko, E. Legin, A. Legin

Research output

7 Citations (Scopus)

Abstract

© 2016 Elsevier B.V.Fermentation process monitoring is a very important factor for controlled protein biosynthesis in industry. Currently the only method to determine protein concentration in cultivation broth is ELISA (enzyme-linked immunosorbent assay). Having high sensitivity and specificity, ELISA takes several hours to run, which is not suitable for express monitoring. A recent progress in multisensor systems for chemical analysis (electronic tongues) shows a good promise for application of such systems in fermentation-related tasks. The objective of this work was feasibility study of potentiometric multisensor system as an instrument for indirect assessment of protein production through chemical measurements in a cultivation medium. The samples taken from nine fermentation processes intended for production of recombinant protein A of Staphylococcus aureus (SpA) in Escherichia coli were analyzed with the electronic tongue system comprised of 14 potentiometric sensors. The system was able to monitor the g
Original languageEnglish
Pages (from-to)None
JournalSensors and Actuators, B: Chemical
Volume238
DOIs
Publication statusPublished - 2017

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