Increased tRNA concentration in yeast containing mutant termination translation factors eRF1 and eRF3

G. A. Zhuravleva, S. E. Moskalenko, S. V. Shabel'skaia, M. Philippe, S. G. Inge-Vechtomov

Research output

3 Citations (Scopus)

Abstract

Earlier we have characterized strains bearing mutations in essential genes SUP45 and SUP35 of yeast S. cerevisiae, encoding translation termination factors eRF1 and eRF3 respectively. In the present work nonsense-mutants on genes SUP45 and SUP35 have been compared by a level of eight tRNA: tRNATyr, tRNAGln, tRNATrp, tRNALeu and tRNAArg (previously described as potentially suppressor tRNA), and also tRNAPro, tRNAHis and tRNAGly. We have not revealed preferable increase in amount of natural suppressor tRNA. The majority of the investigated mutations leads to increase in a level of all investigated tRNA. The mechanisms providing viability of nonsense-mutants on essential genes SUP45 and SUP35 are discussed.

Original languageEnglish
Pages (from-to)724-730
Number of pages7
JournalMolekuliarnaia biologiia.
Volume40
Issue number4
Publication statusPublished - 1 Jul 2006

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Transfer RNA
Yeasts
Essential Genes
RNA, Transfer, Gly
RNA, Transfer, His
RNA, Transfer, Arg
RNA, Transfer, Pro
RNA, Transfer, Gln
RNA, Transfer, Tyr
RNA, Transfer, Leu
Mutation
Saccharomyces cerevisiae
Genes

Scopus subject areas

  • Molecular Biology

Cite this

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abstract = "Earlier we have characterized strains bearing mutations in essential genes SUP45 and SUP35 of yeast S. cerevisiae, encoding translation termination factors eRF1 and eRF3 respectively. In the present work nonsense-mutants on genes SUP45 and SUP35 have been compared by a level of eight tRNA: tRNATyr, tRNAGln, tRNATrp, tRNALeu and tRNAArg (previously described as potentially suppressor tRNA), and also tRNAPro, tRNAHis and tRNAGly. We have not revealed preferable increase in amount of natural suppressor tRNA. The majority of the investigated mutations leads to increase in a level of all investigated tRNA. The mechanisms providing viability of nonsense-mutants on essential genes SUP45 and SUP35 are discussed.",
author = "Zhuravleva, {G. A.} and Moskalenko, {S. E.} and Shabel'skaia, {S. V.} and M. Philippe and Inge-Vechtomov, {S. G.}",
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T1 - Increased tRNA concentration in yeast containing mutant termination translation factors eRF1 and eRF3

AU - Zhuravleva, G. A.

AU - Moskalenko, S. E.

AU - Shabel'skaia, S. V.

AU - Philippe, M.

AU - Inge-Vechtomov, S. G.

PY - 2006/7/1

Y1 - 2006/7/1

N2 - Earlier we have characterized strains bearing mutations in essential genes SUP45 and SUP35 of yeast S. cerevisiae, encoding translation termination factors eRF1 and eRF3 respectively. In the present work nonsense-mutants on genes SUP45 and SUP35 have been compared by a level of eight tRNA: tRNATyr, tRNAGln, tRNATrp, tRNALeu and tRNAArg (previously described as potentially suppressor tRNA), and also tRNAPro, tRNAHis and tRNAGly. We have not revealed preferable increase in amount of natural suppressor tRNA. The majority of the investigated mutations leads to increase in a level of all investigated tRNA. The mechanisms providing viability of nonsense-mutants on essential genes SUP45 and SUP35 are discussed.

AB - Earlier we have characterized strains bearing mutations in essential genes SUP45 and SUP35 of yeast S. cerevisiae, encoding translation termination factors eRF1 and eRF3 respectively. In the present work nonsense-mutants on genes SUP45 and SUP35 have been compared by a level of eight tRNA: tRNATyr, tRNAGln, tRNATrp, tRNALeu and tRNAArg (previously described as potentially suppressor tRNA), and also tRNAPro, tRNAHis and tRNAGly. We have not revealed preferable increase in amount of natural suppressor tRNA. The majority of the investigated mutations leads to increase in a level of all investigated tRNA. The mechanisms providing viability of nonsense-mutants on essential genes SUP45 and SUP35 are discussed.

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