In vivo and in vitro application of near-infrared fiber optic probe for Ehrlich carcinoma distinction: Towards the development of real-time tumor margins assessment tool

Ekaterina Oleneva, Andrey Panchenko, Maria Khaydukova, Ekaterina Gubareva, Olga Bibikova, Viacheslav Artyushenko, Andrey Legin, Dmitry Kirsanov

Research output

Abstract

This report describes a full-scale experiment on intradermal Ehrlich carcinoma (EC) differentiation in mouse model using NIR spectroscopy in diffuse reflectance mode and chemometric data processing. EC is widely used as an experimental tumor model due to its resemblance with human undifferentiated epithelial tumors and can be applied as a preclinical testing in order to verify the capability of NIR spectroscopy to distinguish cancer from healthy tissues before a clinical research with an aim of creating a new analytical tool for on-line intraoperative tumor margins assessment. The study consists of five steps of NIR spectra measurements: in vivo on the early stage of carcinoma growth; in vivo on the advanced stage of carcinoma growth; in vivo during the surgery; in vitro study of the post-operative materials stored in formalin; in vitro study of the post-operative materials stored in paraffin. It was shown that reliable tumor differentiation with a compact optic fiber probe was possible in all these cases. The classification models were built on two data sets, obtained during in vivo and in vitro measurements; both of them demonstrated 100% specificity and sensitivity.

Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume213
DOIs
Publication statusPublished - 15 Apr 2019

Fingerprint

Fiber optics
Tumors
margins
fiber optics
tumors
cancer
Infrared radiation
probes
Spectroscopy
Formaldehyde
Paraffin
Paraffins
Surgery
paraffins
surgery
spectroscopy
mice
Tissue
Testing
reflectance

Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

Cite this

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abstract = "This report describes a full-scale experiment on intradermal Ehrlich carcinoma (EC) differentiation in mouse model using NIR spectroscopy in diffuse reflectance mode and chemometric data processing. EC is widely used as an experimental tumor model due to its resemblance with human undifferentiated epithelial tumors and can be applied as a preclinical testing in order to verify the capability of NIR spectroscopy to distinguish cancer from healthy tissues before a clinical research with an aim of creating a new analytical tool for on-line intraoperative tumor margins assessment. The study consists of five steps of NIR spectra measurements: in vivo on the early stage of carcinoma growth; in vivo on the advanced stage of carcinoma growth; in vivo during the surgery; in vitro study of the post-operative materials stored in formalin; in vitro study of the post-operative materials stored in paraffin. It was shown that reliable tumor differentiation with a compact optic fiber probe was possible in all these cases. The classification models were built on two data sets, obtained during in vivo and in vitro measurements; both of them demonstrated 100{\%} specificity and sensitivity.",
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author = "Ekaterina Oleneva and Andrey Panchenko and Maria Khaydukova and Ekaterina Gubareva and Olga Bibikova and Viacheslav Artyushenko and Andrey Legin and Dmitry Kirsanov",
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AU - Gubareva, Ekaterina

AU - Bibikova, Olga

AU - Artyushenko, Viacheslav

AU - Legin, Andrey

AU - Kirsanov, Dmitry

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