TY - JOUR
T1 - In vivo and in vitro application of near-infrared fiber optic probe for Ehrlich carcinoma distinction
T2 - Towards the development of real-time tumor margins assessment tool
AU - Oleneva, Ekaterina
AU - Panchenko, Andrey
AU - Khaydukova, Maria
AU - Gubareva, Ekaterina
AU - Bibikova, Olga
AU - Artyushenko, Viacheslav
AU - Legin, Andrey
AU - Kirsanov, Dmitry
PY - 2019/4/15
Y1 - 2019/4/15
N2 - This report describes a full-scale experiment on intradermal Ehrlich carcinoma (EC) differentiation in mouse model using NIR spectroscopy in diffuse reflectance mode and chemometric data processing. EC is widely used as an experimental tumor model due to its resemblance with human undifferentiated epithelial tumors and can be applied as a preclinical testing in order to verify the capability of NIR spectroscopy to distinguish cancer from healthy tissues before a clinical research with an aim of creating a new analytical tool for on-line intraoperative tumor margins assessment. The study consists of five steps of NIR spectra measurements: in vivo on the early stage of carcinoma growth; in vivo on the advanced stage of carcinoma growth; in vivo during the surgery; in vitro study of the post-operative materials stored in formalin; in vitro study of the post-operative materials stored in paraffin. It was shown that reliable tumor differentiation with a compact optic fiber probe was possible in all these cases. The classification models were built on two data sets, obtained during in vivo and in vitro measurements; both of them demonstrated 100% specificity and sensitivity.
AB - This report describes a full-scale experiment on intradermal Ehrlich carcinoma (EC) differentiation in mouse model using NIR spectroscopy in diffuse reflectance mode and chemometric data processing. EC is widely used as an experimental tumor model due to its resemblance with human undifferentiated epithelial tumors and can be applied as a preclinical testing in order to verify the capability of NIR spectroscopy to distinguish cancer from healthy tissues before a clinical research with an aim of creating a new analytical tool for on-line intraoperative tumor margins assessment. The study consists of five steps of NIR spectra measurements: in vivo on the early stage of carcinoma growth; in vivo on the advanced stage of carcinoma growth; in vivo during the surgery; in vitro study of the post-operative materials stored in formalin; in vitro study of the post-operative materials stored in paraffin. It was shown that reliable tumor differentiation with a compact optic fiber probe was possible in all these cases. The classification models were built on two data sets, obtained during in vivo and in vitro measurements; both of them demonstrated 100% specificity and sensitivity.
KW - Cancer distinction
KW - Ehrlich carcinoma
KW - Near-infrared spectroscopy
KW - Support vector machines
UR - http://www.scopus.com/inward/record.url?scp=85060253811&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2019.01.061
DO - 10.1016/j.saa.2019.01.061
M3 - Article
C2 - 30677734
AN - SCOPUS:85060253811
VL - 213
SP - 12
EP - 18
JO - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
JF - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
SN - 1386-1425
ER -