Imidazolium Ionic Liquids in Microemulsion Electrokinetic Chromatography for Separation of Polyphenol Antioxidants

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Abstract

Analytical prospects of ionic liquids as the components of the microemulsion for polyphenols separation by microemulsion electrokinetic chromatography are explored. Imidazolium-based hydrophilic ionic liquid 1-hexadecyl-3-methylimidazolium chloride was used as a surfactant and hydrophobic ionic liquids 1-hexyl-3-methylimidazolium tetrafluoroborate and 1-hexyl-3-methylimidazolium bis(trifluormethylsulfonyl)imide were used as the oil phase. It was shown that the addition of ionic liquids into the microemulsion leads to a significant increase in the number of theoretical plates for catechins separation (in the range 200–650 ×103) in both cases. It was established that the use of hydrophobic ionic liquids 1-hexyl-3-methylimidazolium tetrafluoroborate and 1-hexyl-3-methylimidazolium bis(trifluormethylsulfonyl)imide increases the sensitivity and selectivity of the polyphenols separation compared to 1-hexadecyl-3-methylimidazolium chloride. The following composition of microemulsion was found to be optimal for the selective electrophoretic separation of catechins with ionic liquids as the oil phase: 0.6% (w/v) sodium dodecyl sulphate, 0.7% (w/v) 1-hexyl-3-methylimidazolium tetrafluoroborate, 1.6% (w/v) 1-butanol, 5 mM phosphate buffer solution, pH = 7. The conditions for the electrophoretic separation of catechins with microemulsion electrokinetic chromatography were used to determine polyphenols in tea samples.

Original languageEnglish
Pages (from-to)1001-1008
Number of pages8
JournalChromatographia
Volume83
Issue number8
Early online date28 Jun 2020
DOIs
StatePublished - 1 Aug 2020

Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Keywords

  • Imidazolium ionic liquids
  • Microemulsion
  • Microemulsion electrokinetic chromatography
  • Polyphenols

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