Research output: Contribution to journal › Article › peer-review
Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carries for Improved Colistin Delivery. / Dubashynskaya , Natallia V. ; Raik, Sergei V. ; Dubrovskii, Yaroslav A. ; Demyanova , Elena V. ; Shcherbakova, Elena S. ; Poshina, Daria N. ; Shasherina, Anna Y. ; Anufrikov, Yuri A. ; Skorik, Yury A. .
In: International Journal of Molecular Sciences, Vol. 22, No. 16, 8381, 02.08.2021, p. 8381-8393.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carries for Improved Colistin Delivery
AU - Dubashynskaya , Natallia V.
AU - Raik, Sergei V.
AU - Dubrovskii, Yaroslav A.
AU - Demyanova , Elena V.
AU - Shcherbakova, Elena S.
AU - Poshina, Daria N.
AU - Shasherina, Anna Y.
AU - Anufrikov, Yuri A.
AU - Skorik, Yury A.
N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/8/2
Y1 - 2021/8/2
N2 - Improving the therapeutic characteristics of antibiotics is an effective strategy for controlling the growth of multidrug-resistant Gram-negative microorganisms. The purpose of this study was to develop a colistin (CT) delivery system based on hyaluronic acid (HA) and the water-soluble cationic chitosan derivative, diethylaminoethyl chitosan (DEAECS). The CT delivery system was a polyelectrolyte complex (PEC) obtained by interpolymeric interactions between the HA polyanion and the DEAECS polycation, with simultaneous inclusion of positively charged CT molecules into the resulting complex. The developed PEC had a hydrodynamic diameter of 210–250 nm and a negative surface charge (ζ-potential = −19 mV); the encapsulation and loading efficiencies were 100 and 16.7%, respectively. The developed CT delivery systems were characterized by modified release (30–40% and 85–90% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro experiments showed that the encapsulation of CT in polysaccharide carriers did not reduce its antimicrobial activity, as the minimum inhibitory concentrations against Pseudomonas aeruginosa of both encapsulated CT and pure CT were 1 μg/mL.
AB - Improving the therapeutic characteristics of antibiotics is an effective strategy for controlling the growth of multidrug-resistant Gram-negative microorganisms. The purpose of this study was to develop a colistin (CT) delivery system based on hyaluronic acid (HA) and the water-soluble cationic chitosan derivative, diethylaminoethyl chitosan (DEAECS). The CT delivery system was a polyelectrolyte complex (PEC) obtained by interpolymeric interactions between the HA polyanion and the DEAECS polycation, with simultaneous inclusion of positively charged CT molecules into the resulting complex. The developed PEC had a hydrodynamic diameter of 210–250 nm and a negative surface charge (ζ-potential = −19 mV); the encapsulation and loading efficiencies were 100 and 16.7%, respectively. The developed CT delivery systems were characterized by modified release (30–40% and 85–90% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro experiments showed that the encapsulation of CT in polysaccharide carriers did not reduce its antimicrobial activity, as the minimum inhibitory concentrations against Pseudomonas aeruginosa of both encapsulated CT and pure CT were 1 μg/mL.
KW - Antimicrobial activity
KW - Colistin
KW - Diethylaminoethyl chitosan
KW - Drug delivery system
KW - ESKAPE pathogens
KW - Hyaluronic acid
KW - Polyelectrolyte complexes
KW - Polymyxin
KW - Hyaluronic Acid/chemistry
KW - Anti-Bacterial Agents/administration & dosage
KW - Humans
KW - Colistin/administration & dosage
KW - Drug Carriers/chemistry
KW - Pseudomonas Infections/drug therapy
KW - Polyelectrolytes/chemistry
KW - Chitosan/chemistry
KW - Pseudomonas aeruginosa/drug effects
KW - BIODEGRADATION
KW - polymyxin
KW - DRUG-DELIVERY
KW - NANOCARRIERS
KW - diethylaminoethyl chitosan
KW - HYALURONIC-ACID
KW - hyaluronic acid
KW - DERIVATIVES
KW - colistin
KW - MECHANISMS
KW - antimicrobial activity
KW - FUNCTIONALIZATION
KW - NANOPARTICLES
KW - polyelectrolyte complexes
KW - drug delivery system
UR - http://www.scopus.com/inward/record.url?scp=85111728802&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/180a33e3-c83a-3f4a-a605-a73b7bf8b23d/
U2 - 10.3390/ijms22168381
DO - 10.3390/ijms22168381
M3 - Article
C2 - 34445088
VL - 22
SP - 8381
EP - 8393
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 16
M1 - 8381
ER -
ID: 87286469