HPLC analysis of synthetic polymers on short monolithic columns

Research output

10 Citations (Scopus)

Abstract

Ultrashort monolithic columns (disks) were thoroughly studied as efficient stationary phases for precipitation-dissolution chromatography of synthetic polymers. Gradient elution mode was applied in all chromatographic runs. The mixtures of different flexible chain homopolymers, such as polystyrenes, poly(methyl methacrylates), and poly(tert-butylmethacrylates) were separated according to their molecular weights on both commercial poly(styrene-co- divinylbenzene) disks (12 id × 3 mm and 5 × 5 mm) and lab-made monolithic columns (4.6 id × 50 mm) filled with supports of different hydrophobicity. The experimental conditions were optimized to reach fast and highly efficient separation. It was observed that, similar to the separation of monoliths of other classes of (macro)molecules (proteins, DNA, oligonucleotides), the length of column did not affect the peak resolution. A comparison of the retention properties of the poly(styrene-co-divinylbenzene) disk-shaped monoliths with those based on poly(lauryl methacryla
Original languageEnglish
Pages (from-to)3741-3749
JournalJournal of Separation Science
Issue number23
DOIs
Publication statusPublished - 2013

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Styrene
Polymers
Oligonucleotides
Polystyrenes
Polymethyl Methacrylate
Hydrophobicity
Chromatography
Homopolymerization
Polymethyl methacrylates
Macros
Dissolution
DNA
Molecular weight
Proteins
Molecules
divinylbenzene-polystyrene copolymer

Cite this

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title = "HPLC analysis of synthetic polymers on short monolithic columns",
abstract = "Ultrashort monolithic columns (disks) were thoroughly studied as efficient stationary phases for precipitation-dissolution chromatography of synthetic polymers. Gradient elution mode was applied in all chromatographic runs. The mixtures of different flexible chain homopolymers, such as polystyrenes, poly(methyl methacrylates), and poly(tert-butylmethacrylates) were separated according to their molecular weights on both commercial poly(styrene-co- divinylbenzene) disks (12 id × 3 mm and 5 × 5 mm) and lab-made monolithic columns (4.6 id × 50 mm) filled with supports of different hydrophobicity. The experimental conditions were optimized to reach fast and highly efficient separation. It was observed that, similar to the separation of monoliths of other classes of (macro)molecules (proteins, DNA, oligonucleotides), the length of column did not affect the peak resolution. A comparison of the retention properties of the poly(styrene-co-divinylbenzene) disk-shaped monoliths with those based on poly(lauryl methacryla",
author = "E. Maksimova and E. Vlakh and E. Sinitsyna and T. Tennikova",
year = "2013",
doi = "10.1002/jssc.201300852",
language = "English",
pages = "3741--3749",
journal = "Journal of Separation Science",
issn = "1615-9306",
publisher = "Wiley-Blackwell",
number = "23",

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TY - JOUR

T1 - HPLC analysis of synthetic polymers on short monolithic columns

AU - Maksimova, E.

AU - Vlakh, E.

AU - Sinitsyna, E.

AU - Tennikova, T.

PY - 2013

Y1 - 2013

N2 - Ultrashort monolithic columns (disks) were thoroughly studied as efficient stationary phases for precipitation-dissolution chromatography of synthetic polymers. Gradient elution mode was applied in all chromatographic runs. The mixtures of different flexible chain homopolymers, such as polystyrenes, poly(methyl methacrylates), and poly(tert-butylmethacrylates) were separated according to their molecular weights on both commercial poly(styrene-co- divinylbenzene) disks (12 id × 3 mm and 5 × 5 mm) and lab-made monolithic columns (4.6 id × 50 mm) filled with supports of different hydrophobicity. The experimental conditions were optimized to reach fast and highly efficient separation. It was observed that, similar to the separation of monoliths of other classes of (macro)molecules (proteins, DNA, oligonucleotides), the length of column did not affect the peak resolution. A comparison of the retention properties of the poly(styrene-co-divinylbenzene) disk-shaped monoliths with those based on poly(lauryl methacryla

AB - Ultrashort monolithic columns (disks) were thoroughly studied as efficient stationary phases for precipitation-dissolution chromatography of synthetic polymers. Gradient elution mode was applied in all chromatographic runs. The mixtures of different flexible chain homopolymers, such as polystyrenes, poly(methyl methacrylates), and poly(tert-butylmethacrylates) were separated according to their molecular weights on both commercial poly(styrene-co- divinylbenzene) disks (12 id × 3 mm and 5 × 5 mm) and lab-made monolithic columns (4.6 id × 50 mm) filled with supports of different hydrophobicity. The experimental conditions were optimized to reach fast and highly efficient separation. It was observed that, similar to the separation of monoliths of other classes of (macro)molecules (proteins, DNA, oligonucleotides), the length of column did not affect the peak resolution. A comparison of the retention properties of the poly(styrene-co-divinylbenzene) disk-shaped monoliths with those based on poly(lauryl methacryla

U2 - 10.1002/jssc.201300852

DO - 10.1002/jssc.201300852

M3 - Article

SP - 3741

EP - 3749

JO - Journal of Separation Science

JF - Journal of Separation Science

SN - 1615-9306

IS - 23

ER -