Horseradish Peroxidase-Based Biosensors with Different Nanotransducers for the Determination of Hydrogen Peroxide

K. G. Nikolaev, S. S. Ermakov, Yu E. Ermolenko, D. V. Navolotskaya, A. Offenhäusser, Yu G. Mourzina

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: We studied methods of the in situ synthesis of gold nanostructures on the electrode surface and of the directed electrochemical synthesis of nanodendrites to create biosensors based on horseradish peroxidase. The effect of nanostructuring of the transducer surface and its subsequent modification on the electroanalytical characteristics of sensors for the determination of hydrogen peroxide was studied. The use of these methods can significantly improve the performance characteristics of the biosensors, such as the sensitivity coefficient, limit of detection, and stability in the range of micromolar and submicromolar concentrations of hydrogen peroxide. The electrodes modified by the in situ synthesis of nanostructures with subsequent treatment with Meerwein’s reagent, as well as ultramicroelectrodes prepared by directional electrochemical synthesis, show low limits of detection for hydrogen peroxide, 0.2 and 0.08 µM, and high sensitivity coefficients, 0.71 and 0.84 A M–1 cm–2, respectively.

Original languageEnglish
Pages (from-to)510-517
Number of pages8
JournalJournal of Analytical Chemistry
Volume76
Issue number4
DOIs
StatePublished - Apr 2021

Scopus subject areas

  • Analytical Chemistry

Keywords

  • electrochemical biosensor
  • enzyme
  • gold nanostructures
  • hydrogen peroxide
  • modified electrode
  • peroxidase

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