When applied only to the outer surface of the isolated frog skin furosemide caused a prompt, dose-related (0.1--1.0 mg/ml) and fully reversible increase in potential difference; it did not change the short-circuit current, however. Potential differences were not altered by furosemide in the Ringer-sulphate solution. Furosemide-induced increase of potential differences could be reversed by nystatine which increased the ionic permeability. These results suggest that furosemide could decrease the passive entrance of chlorides through the barrier of the apical cell membrane of the frog skin.
|Translated title of the contribution||Hyperpolarization of frog skin exposed to furosemide|
|Number of pages||3|
|Journal||Byulleten Eksperimentalnoi Biologii i Meditsiny|
|State||Published - 1 Jun 1975|
Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)