Genetic Basis of Attenuation of Cold-Adapted Influenza Strain B/Leningrad/14/17/55 — Backup Master Donor Virus for Influenza Type B Live Attenuated Vaccines

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Genetic Basis of Attenuation of Cold-Adapted Influenza Strain B/Leningrad/14/17/55 — Backup Master Donor Virus for Influenza Type B Live Attenuated Vaccines. / Krutikova, E. V.; Stepanova, E. A.; Wong, P. F.; Kiseleva, I. V.; Grigor’eva, E. P.; Rudenko, L. G.

In: Bulletin of Experimental Biology and Medicine, Vol. 168, No. 5, 01.03.2020, p. 669-672.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{ab62986664684835b492646500b7931c,

title = "Genetic Basis of Attenuation of Cold-Adapted Influenza Strain B/Leningrad/14/17/55 — Backup Master Donor Virus for Influenza Type B Live Attenuated Vaccines",

abstract = "The reassortant vaccine strain of live attenuated influenza vaccine inherits temperature sensitivity and areactogenicity from cold-adapted attenuated master donor virus. In Russia, B/ USSR/60/69 master donor virus (B60) is currently in use for the preparation of live attenuated type B influenza vaccine candidates. Trivalent live attenuated influenza vaccine based on A/ Leningrad/134/17/57 and B60 are licensed for the use in Russia for single dose vaccination of adults and children over 3 years. B/Leningrad/14/17/55 (B14) cold-adapted virus is a backup master donor virus for live attenuated type B influenza vaccine. According to our preliminary estimates, it is more attenuated than B60, which can allow expanding applicability of this vaccine for children under 3 years of age. In this paper, the role of B14 genes in its attenuation was assessed. Representative collection of reassortants of B14 with epidemic influenza B viruses was obtained, a phenotypic analysis of reassortants was performed, and their pathogenicity for animals was assessed. The leading role of PB2 and PA genes in attenuation of B14 master donor virus was proven.",

keywords = "attenuation, influenza В virus, live attenuated influenza vaccine, master donor virus",

author = "Krutikova, {E. V.} and Stepanova, {E. A.} and Wong, {P. F.} and Kiseleva, {I. V.} and Grigor{\textquoteright}eva, {E. P.} and Rudenko, {L. G.}",

year = "2020",

month = mar,

day = "1",

doi = "10.1007/s10517-020-04777-x",

language = "English",

volume = "168",

pages = "669--672",

journal = "Bulletin of Experimental Biology and Medicine",

issn = "0007-4888",

publisher = "Springer Nature",

number = "5",

}

RIS

TY - JOUR

T1 - Genetic Basis of Attenuation of Cold-Adapted Influenza Strain B/Leningrad/14/17/55 — Backup Master Donor Virus for Influenza Type B Live Attenuated Vaccines

AU - Krutikova, E. V.

AU - Stepanova, E. A.

AU - Wong, P. F.

AU - Kiseleva, I. V.

AU - Grigor’eva, E. P.

AU - Rudenko, L. G.

PY - 2020/3/1

Y1 - 2020/3/1

N2 - The reassortant vaccine strain of live attenuated influenza vaccine inherits temperature sensitivity and areactogenicity from cold-adapted attenuated master donor virus. In Russia, B/ USSR/60/69 master donor virus (B60) is currently in use for the preparation of live attenuated type B influenza vaccine candidates. Trivalent live attenuated influenza vaccine based on A/ Leningrad/134/17/57 and B60 are licensed for the use in Russia for single dose vaccination of adults and children over 3 years. B/Leningrad/14/17/55 (B14) cold-adapted virus is a backup master donor virus for live attenuated type B influenza vaccine. According to our preliminary estimates, it is more attenuated than B60, which can allow expanding applicability of this vaccine for children under 3 years of age. In this paper, the role of B14 genes in its attenuation was assessed. Representative collection of reassortants of B14 with epidemic influenza B viruses was obtained, a phenotypic analysis of reassortants was performed, and their pathogenicity for animals was assessed. The leading role of PB2 and PA genes in attenuation of B14 master donor virus was proven.

AB - The reassortant vaccine strain of live attenuated influenza vaccine inherits temperature sensitivity and areactogenicity from cold-adapted attenuated master donor virus. In Russia, B/ USSR/60/69 master donor virus (B60) is currently in use for the preparation of live attenuated type B influenza vaccine candidates. Trivalent live attenuated influenza vaccine based on A/ Leningrad/134/17/57 and B60 are licensed for the use in Russia for single dose vaccination of adults and children over 3 years. B/Leningrad/14/17/55 (B14) cold-adapted virus is a backup master donor virus for live attenuated type B influenza vaccine. According to our preliminary estimates, it is more attenuated than B60, which can allow expanding applicability of this vaccine for children under 3 years of age. In this paper, the role of B14 genes in its attenuation was assessed. Representative collection of reassortants of B14 with epidemic influenza B viruses was obtained, a phenotypic analysis of reassortants was performed, and their pathogenicity for animals was assessed. The leading role of PB2 and PA genes in attenuation of B14 master donor virus was proven.

KW - attenuation

KW - influenza В virus

KW - live attenuated influenza vaccine

KW - master donor virus

UR - http://www.scopus.com/inward/record.url?scp=85083658629&partnerID=8YFLogxK

U2 - 10.1007/s10517-020-04777-x

DO - 10.1007/s10517-020-04777-x

M3 - Article

C2 - 32248454

AN - SCOPUS:85083658629

VL - 168

SP - 669

EP - 672

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

SN - 0007-4888

IS - 5

ER -

ID: 75081981