Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val.

Polina Klauzen, Kseniya Perepelina, Aleksandr Khudiakov, Anna Zlotina, Yulia Fomicheva, Tatiana Pervunina, Tatiana Vershinina, Anna Kostareva, Anna Malashicheva

Research output

Abstract

LMNA mutations are often linked to laminopathies characterized by tissue-specific disorders. We generated two induced pluripotent stem cells lines from patient carrying genetic variant LMNA p.Asp357Val associated with paroxysmal ventricular tachycardia and myopathy. Reprogramming of patient's peripheral blood mononuclear cells was performed using Sendai viruses. Characterization of the FAMRCi005-A and FAMRCi005-B lines revealed that generated iPSC lines expressed pluripotent stem cell markers, had normal karyotype and demonstrated triliniage differentiation ability. Generated cell lines can be used to investigate the molecular links between LMNA genetic variants and cardiac disorders.

Original languageEnglish
Article number101719
JournalStem Cell Research
Volume43
DOIs
Publication statusPublished - Mar 2020

Scopus subject areas

  • Developmental Biology
  • Cell Biology

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