Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene

Aleksandr Khudiakov, Daria Kostina, Anna Zlotina, Tatiana Nikulina, Alexey Sergushichev, Alexandra Gudkova, Alexey Tomilin, Anna Malashicheva, Anna Kostareva

Research output

3 Citations (Scopus)

Abstract

Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalStem Cell Research
Volume24
DOIs
Publication statusPublished - 1 Oct 2017

Scopus subject areas

  • Developmental Biology
  • Cell Biology

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