Functionalization of polyacrylamide for nanotrapping positively charged biomolecules

Nadejda Davydova, Rodriguez Xavier, Carlos Blazquez, Andres Gomez, Igor Perevyazko, Judith Guasch, Vladimir Sergeev, Elena Laukhina, Imma Ratera, Jaume Veciana

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is crucial in order to achieve earlier diagnostics in different diseases. This article demonstrates that using free radical copolymerization, polyacrylamide can be successfully functionalized with specific synthons for nanotrapping positively charged molecules, such as numerous proteins, through electrostatic interactions due to their negative charge. Specifically, two functional random copolymers, acrylamide/acrylic acid (1) and acrylamide/acrylic acid/N-(pyridin-4-yl-methyl)acrylamide (2), whose negative net charges differ in their water solutions, were synthetized and their ability to trap positively charged proteins was studied using myoglobin as a proof-of-concept example. In aqueous solutions, copolymer 1, whose net charge for a 100 chain fragment (Q pH 6/M) is -1.323 × 10 -3, interacted with myoglobin forming a stable monodisperse nanosuspension. In contrast, copolymer 2, whose value of Q pH 6/M equals -0.361 × 10 -3, was not able to form stable particles with myoglobin. Nevertheless, thin films of both copolymers were grown using a dewetting process, which exhibited nanoscale cavities capable of trapping different amounts of myoglobin, as demonstrated by bimodal AFM imaging. The simple procedures used to build protein traps make this engineering approach promising for the development of new materials for biomedical applications where trapping biomolecules is required.

Original languageEnglish
Pages (from-to)15402-15409
Number of pages8
JournalRSC Advances
Volume9
Issue number27
DOIs
StatePublished - 19 May 2019

Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Keywords

  • DNA
  • FILMS
  • MARKER
  • MYOGLOBIN
  • OPTICAL MANIPULATION
  • PARTICLE
  • TRAP

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