Frameshift suppression through inactivation of translation termination in yeast Saccharomyces cerevisiae: Significance of the local context

N. A. Ryabinkova, Yu V. Sopova, G. V. Polozkov, M. V. Savelova, S. G. Inge-Vechtomov

Research output

Abstract

Site-directed mutagenesis and nucleotide sequence analysis were used to study the roles of the global and local contexts in suppression of the lys2-90 frameshift (FS) mutation in Saccharomyces cerevisiae. Global context features established for the LYS2 mRNA region containing the extra T (lys2-90) were similar to those characteristic of regions involved in translational frameshifting. These were a potential ability of the region to form a pseudoknot and the presence of heptanucleotide CUU UGA C with the "hungry" UGA nonsense codon in the pseudoknot. Some local context features proved to be essential for the phenotypic expression of FS suppression as a result of translational frameshifting. Two amino acid substitutions determined by the nucleotide sequence between the extra U and the UGA nonsense codon lacked expression. A dependence was observed between the efficiency of FS suppression and the type of the nonsense codon located at a particular position downstream of the extra nucleotide (UGA > UAG > UAA). When translation termination was inactivated, nonsense suppression and FS suppression correlated with each other. These results suggest that translational frameshifting, which underlies suppression in the case of inactivation of translation termination, most likely takes place on the nonsense codon arising as a result of insertion of an extra nucleotide.

Original languageEnglish
Pages (from-to)717-722
Number of pages6
JournalRussian Journal of Genetics
Volume40
Issue number7
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

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Ribosomal Frameshifting
Nonsense Codon
Saccharomyces cerevisiae
Yeasts
Terminator Codon
Nucleotides
Frameshift Mutation
Amino Acid Substitution
Site-Directed Mutagenesis
Sequence Analysis
Messenger RNA

Scopus subject areas

  • Genetics

Cite this

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title = "Frameshift suppression through inactivation of translation termination in yeast Saccharomyces cerevisiae: Significance of the local context",
abstract = "Site-directed mutagenesis and nucleotide sequence analysis were used to study the roles of the global and local contexts in suppression of the lys2-90 frameshift (FS) mutation in Saccharomyces cerevisiae. Global context features established for the LYS2 mRNA region containing the extra T (lys2-90) were similar to those characteristic of regions involved in translational frameshifting. These were a potential ability of the region to form a pseudoknot and the presence of heptanucleotide CUU UGA C with the {"}hungry{"} UGA nonsense codon in the pseudoknot. Some local context features proved to be essential for the phenotypic expression of FS suppression as a result of translational frameshifting. Two amino acid substitutions determined by the nucleotide sequence between the extra U and the UGA nonsense codon lacked expression. A dependence was observed between the efficiency of FS suppression and the type of the nonsense codon located at a particular position downstream of the extra nucleotide (UGA > UAG > UAA). When translation termination was inactivated, nonsense suppression and FS suppression correlated with each other. These results suggest that translational frameshifting, which underlies suppression in the case of inactivation of translation termination, most likely takes place on the nonsense codon arising as a result of insertion of an extra nucleotide.",
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AU - Inge-Vechtomov, S. G.

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