Extracellular MicroRNAs and Mitochondrial DNA as Potential Biomarkers of Arrhythmogenic Cardiomyopathy

A. A. Khudiakov, N. A. Smolina, K. I. Perepelina, A. B. Malashicheva, A. A. Kostareva

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Differential diagnosis of arrhythmogenic cardiomyopathy (ACM) during the disease latent phase is a challenging clinical problem that requires identification of early ACM biomarkers. Because extracellular nucleic acids are stable, specific, and can be easily detected, they can be used as reliable biomarkers of various diseases. In this study, we analyzed the levels of extracellular microRNAs and mitochondrial DNA in the conditioned medium collected from cardiomyocytes differentiated from induced pluripotent stem cells of ACM patients and healthy donor. Several microRNAs were expressed differently by the affected and healthy cardiomyocytes; therefore, they could be considered as potential ACM biomarkers.

Original languageEnglish
Pages (from-to)272-282
Number of pages11
JournalBiochemistry (Moscow)
Volume84
Issue number3
DOIs
StatePublished - 1 Mar 2019

Keywords

  • arrhythmogenic cardiomyopathy
  • cardiomyocytes
  • induced pluripotent stem cells
  • microRNA
  • mitochondrial DNA

Scopus subject areas

  • Biochemistry

Cite this

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Extracellular MicroRNAs and Mitochondrial DNA as Potential Biomarkers of Arrhythmogenic Cardiomyopathy. / Khudiakov, A. A.; Smolina, N. A.; Perepelina, K. I.; Malashicheva, A. B.; Kostareva, A. A.

In: Biochemistry (Moscow), Vol. 84, No. 3, 01.03.2019, p. 272-282.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Extracellular MicroRNAs and Mitochondrial DNA as Potential Biomarkers of Arrhythmogenic Cardiomyopathy

AU - Khudiakov, A. A.

AU - Smolina, N. A.

AU - Perepelina, K. I.

AU - Malashicheva, A. B.

AU - Kostareva, A. A.

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AB - Differential diagnosis of arrhythmogenic cardiomyopathy (ACM) during the disease latent phase is a challenging clinical problem that requires identification of early ACM biomarkers. Because extracellular nucleic acids are stable, specific, and can be easily detected, they can be used as reliable biomarkers of various diseases. In this study, we analyzed the levels of extracellular microRNAs and mitochondrial DNA in the conditioned medium collected from cardiomyocytes differentiated from induced pluripotent stem cells of ACM patients and healthy donor. Several microRNAs were expressed differently by the affected and healthy cardiomyocytes; therefore, they could be considered as potential ACM biomarkers.

KW - arrhythmogenic cardiomyopathy

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