Exploring the dual impact of hydrocarbon chainlength and the role of piroxicam a conventional NSAID on soylecithin/ion pair amphiphiles mediated hybrid vesicles for brain – tumor targeted drug delivery

Pritam Guha, Biplab Roy, Prasant Nahak, Gourab Karmakar, Chien H. Chang, Алексей Геннадьевич Быков, Александр Владимирович Акентьев, Борис Анатольевич Носков, Amit Kumar Mandal, Anoop Kumar, P.A. Hassan, V.K. Aswal, Takeshi Misono, Kanjiro Torigoe, Amiya Kumar Panda

Research output

Abstract

Development of drug delivery systems, not the drug discovery, has become more cynosures towards the efficacy of drug against the target cells. Modified hybrid cationic vesicles (HCV) were formulated using soylecithin (SLC), ion pair amphiphile (IPA, hexadecyltrimethylammonium-dodecylsulfate, HTMA+-DS−), bi-tail cationic surfactant dialkyldimethylammonium bromides (DXDABs, bis-C12 to C18) and cholesterol. Piroxicam (Px), a conventional non steroidal anti inflamatory drug (NSAID), with potent yet unexplored anticancer activity, was encapsulated in the hybrid vesicles. Dual impact of DXDABs and Px on SLC/IPA were scrutinized in the form of monolayer, bilayer and solid supported bilayer. Favourable hydrophobic inteaction between SLC/IPA anddihexadecyldimethylammonium bromide (DHDAB) as well as the intercalation of Px molecules between the amphiphiles were noticed through the surface pressure area measuremnts. Vesicles without and with Px were fairly monodisperse with positive zeta potential (Z. P.) and considerably stable upto two months. Size of the vesicles enhanced with Px incorporation. Vesicles maintain spherical morphology as revealed from the electronic microscopic studies. Differential scanning calaorimetry and FTIR studies confirm the location of Px membrane palisade that enhances the extent of hydration by increasing the proportion of H-bonding. Bilayer thickness and the spacing between two adjecent lamellar phase were investigated by combined small angle neutron scattering and small angle X-ray scattering. Atomic force microscopic studies confirm the Px induced fluidization of membrane bilayer. The entrapment efficiency of vesicles to host Px depends on the amount of IPA present in the bilayer. Px hosted cationic vesicles showed less than 2% hemolysis. The drug reigned supreme over human neuroblastoma cell line (SH-SY 5Y) when encapsulated inside the membrane and was non toxic to normal human blood cell lymphocyte (PBMC) as revealed from cytotoxicity assay.
Original languageEnglish
Article numberGB8CM
Pages (from-to)334–345
Number of pages12
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume546
DOIs
Publication statusPublished - May 2018

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Piroxicam
Amphiphiles
Hydrocarbons
brain
Tumors
Brain
delivery
drugs
tumors
hydrocarbons
Ions
Membranes
Cells
Pharmaceutical Preparations
ions
membranes
Lymphocytes
Cationic surfactants
Cholesterol
Fluidization

Scopus subject areas

  • Chemistry(all)

Cite this

@article{254cfb6de25e478ea1b72dd75daa58a0,
title = "Exploring the dual impact of hydrocarbon chainlength and the role of piroxicam a conventional NSAID on soylecithin/ion pair amphiphiles mediated hybrid vesicles for brain – tumor targeted drug delivery",
abstract = "Development of drug delivery systems, not the drug discovery, has become more cynosures towards the efficacy of drug against the target cells. Modified hybrid cationic vesicles (HCV) were formulated using soylecithin (SLC), ion pair amphiphile (IPA, hexadecyltrimethylammonium-dodecylsulfate, HTMA+-DS−), bi-tail cationic surfactant dialkyldimethylammonium bromides (DXDABs, bis-C12 to C18) and cholesterol. Piroxicam (Px), a conventional non steroidal anti inflamatory drug (NSAID), with potent yet unexplored anticancer activity, was encapsulated in the hybrid vesicles. Dual impact of DXDABs and Px on SLC/IPA were scrutinized in the form of monolayer, bilayer and solid supported bilayer. Favourable hydrophobic inteaction between SLC/IPA anddihexadecyldimethylammonium bromide (DHDAB) as well as the intercalation of Px molecules between the amphiphiles were noticed through the surface pressure area measuremnts. Vesicles without and with Px were fairly monodisperse with positive zeta potential (Z. P.) and considerably stable upto two months. Size of the vesicles enhanced with Px incorporation. Vesicles maintain spherical morphology as revealed from the electronic microscopic studies. Differential scanning calaorimetry and FTIR studies confirm the location of Px membrane palisade that enhances the extent of hydration by increasing the proportion of H-bonding. Bilayer thickness and the spacing between two adjecent lamellar phase were investigated by combined small angle neutron scattering and small angle X-ray scattering. Atomic force microscopic studies confirm the Px induced fluidization of membrane bilayer. The entrapment efficiency of vesicles to host Px depends on the amount of IPA present in the bilayer. Px hosted cationic vesicles showed less than 2{\%} hemolysis. The drug reigned supreme over human neuroblastoma cell line (SH-SY 5Y) when encapsulated inside the membrane and was non toxic to normal human blood cell lymphocyte (PBMC) as revealed from cytotoxicity assay.",
keywords = "ANGLE NEUTRON-SCATTERING; X-RAY-SCATTERING; BILAYER THICKNESS; PHOSPHATIDYLCHOLINE VESICLES; CATIONIC SURFACTANT; CATANIONIC VESICLES; LANGMUIR MONOLAYER; CONTRAST VARIATION; LIPOSOMES; MEMBRANES, Bilayer; Monolayer; Piroxicam; SANS; AFM; MTT assay",
author = "Pritam Guha and Biplab Roy and Prasant Nahak and Gourab Karmakar and Chang, {Chien H.} and Быков, {Алексей Геннадьевич} and Акентьев, {Александр Владимирович} and Носков, {Борис Анатольевич} and Mandal, {Amit Kumar} and Anoop Kumar and P.A. Hassan and V.K. Aswal and Takeshi Misono and Kanjiro Torigoe and Panda, {Amiya Kumar}",
year = "2018",
month = "5",
doi = "10.1016/j.colsurfa.2018.03.025",
language = "English",
volume = "546",
pages = "334–345",
journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",
issn = "0927-7757",
publisher = "Elsevier",

}

TY - JOUR

T1 - Exploring the dual impact of hydrocarbon chainlength and the role of piroxicam a conventional NSAID on soylecithin/ion pair amphiphiles mediated hybrid vesicles for brain – tumor targeted drug delivery

AU - Guha, Pritam

AU - Roy, Biplab

AU - Nahak, Prasant

AU - Karmakar, Gourab

AU - Chang, Chien H.

AU - Быков, Алексей Геннадьевич

AU - Акентьев, Александр Владимирович

AU - Носков, Борис Анатольевич

AU - Mandal, Amit Kumar

AU - Kumar, Anoop

AU - Hassan, P.A.

AU - Aswal, V.K.

AU - Misono, Takeshi

AU - Torigoe, Kanjiro

AU - Panda, Amiya Kumar

PY - 2018/5

Y1 - 2018/5

N2 - Development of drug delivery systems, not the drug discovery, has become more cynosures towards the efficacy of drug against the target cells. Modified hybrid cationic vesicles (HCV) were formulated using soylecithin (SLC), ion pair amphiphile (IPA, hexadecyltrimethylammonium-dodecylsulfate, HTMA+-DS−), bi-tail cationic surfactant dialkyldimethylammonium bromides (DXDABs, bis-C12 to C18) and cholesterol. Piroxicam (Px), a conventional non steroidal anti inflamatory drug (NSAID), with potent yet unexplored anticancer activity, was encapsulated in the hybrid vesicles. Dual impact of DXDABs and Px on SLC/IPA were scrutinized in the form of monolayer, bilayer and solid supported bilayer. Favourable hydrophobic inteaction between SLC/IPA anddihexadecyldimethylammonium bromide (DHDAB) as well as the intercalation of Px molecules between the amphiphiles were noticed through the surface pressure area measuremnts. Vesicles without and with Px were fairly monodisperse with positive zeta potential (Z. P.) and considerably stable upto two months. Size of the vesicles enhanced with Px incorporation. Vesicles maintain spherical morphology as revealed from the electronic microscopic studies. Differential scanning calaorimetry and FTIR studies confirm the location of Px membrane palisade that enhances the extent of hydration by increasing the proportion of H-bonding. Bilayer thickness and the spacing between two adjecent lamellar phase were investigated by combined small angle neutron scattering and small angle X-ray scattering. Atomic force microscopic studies confirm the Px induced fluidization of membrane bilayer. The entrapment efficiency of vesicles to host Px depends on the amount of IPA present in the bilayer. Px hosted cationic vesicles showed less than 2% hemolysis. The drug reigned supreme over human neuroblastoma cell line (SH-SY 5Y) when encapsulated inside the membrane and was non toxic to normal human blood cell lymphocyte (PBMC) as revealed from cytotoxicity assay.

AB - Development of drug delivery systems, not the drug discovery, has become more cynosures towards the efficacy of drug against the target cells. Modified hybrid cationic vesicles (HCV) were formulated using soylecithin (SLC), ion pair amphiphile (IPA, hexadecyltrimethylammonium-dodecylsulfate, HTMA+-DS−), bi-tail cationic surfactant dialkyldimethylammonium bromides (DXDABs, bis-C12 to C18) and cholesterol. Piroxicam (Px), a conventional non steroidal anti inflamatory drug (NSAID), with potent yet unexplored anticancer activity, was encapsulated in the hybrid vesicles. Dual impact of DXDABs and Px on SLC/IPA were scrutinized in the form of monolayer, bilayer and solid supported bilayer. Favourable hydrophobic inteaction between SLC/IPA anddihexadecyldimethylammonium bromide (DHDAB) as well as the intercalation of Px molecules between the amphiphiles were noticed through the surface pressure area measuremnts. Vesicles without and with Px were fairly monodisperse with positive zeta potential (Z. P.) and considerably stable upto two months. Size of the vesicles enhanced with Px incorporation. Vesicles maintain spherical morphology as revealed from the electronic microscopic studies. Differential scanning calaorimetry and FTIR studies confirm the location of Px membrane palisade that enhances the extent of hydration by increasing the proportion of H-bonding. Bilayer thickness and the spacing between two adjecent lamellar phase were investigated by combined small angle neutron scattering and small angle X-ray scattering. Atomic force microscopic studies confirm the Px induced fluidization of membrane bilayer. The entrapment efficiency of vesicles to host Px depends on the amount of IPA present in the bilayer. Px hosted cationic vesicles showed less than 2% hemolysis. The drug reigned supreme over human neuroblastoma cell line (SH-SY 5Y) when encapsulated inside the membrane and was non toxic to normal human blood cell lymphocyte (PBMC) as revealed from cytotoxicity assay.

KW - ANGLE NEUTRON-SCATTERING; X-RAY-SCATTERING; BILAYER THICKNESS; PHOSPHATIDYLCHOLINE VESICLES; CATIONIC SURFACTANT; CATANIONIC VESICLES; LANGMUIR MONOLAYER; CONTRAST VARIATION; LIPOSOMES; MEMBRANES

KW - Bilayer; Monolayer; Piroxicam; SANS; AFM; MTT assay

U2 - 10.1016/j.colsurfa.2018.03.025

DO - 10.1016/j.colsurfa.2018.03.025

M3 - Article

VL - 546

SP - 334

EP - 345

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

M1 - GB8CM

ER -