Effects of short-term drought on metabolism and nutritional properties of pea (Pisum sativum) seeds

T. Leonova, V. Chanceva, O. Shiroglazova, M. Glomb, C. Henning, K. Antonova, A. Soboleva, A. Tsarev, I. Shumilina, E. Dinastiya, T. Baldensperger, G. Smolikova, S. Medvedev, T. Bilova, L. Wessjohann, A. Frolov

Research output

Abstract

Drought is one of the major environmental stressors, affecting crop productivity. Plant response to dehydration is accompanied with metabolic chamges, oxidative stress and enhanced generation of advanced glycation end products (AGEs), which are known to be pro­inflammatory in mammals, impacting on the pathogenesis of diabetes mellitus and ageing. The protein­rich seeds of legumes might be a target of such deleterious changes. However, the effects of drought­related protein damage on the quality of crops, as well as its influence on human health are still unstudied. Therefore, here we address the changes in seed quality, metabolome, protein AGE contents and nutritional properties of pea accompanying short­term drought, applied at the step of seed maturation. For this, pea plants were grown on vermiculite for six weeks before transfer to aqueous medium, to which five days later polyethylene glycol 8000 (PEG­8000) was supplemented. Two days later, the plants were transferred to PEG­free substrate and grown till the end of seed maturation. After evaluation of seed quality, seed metabolites were analyzed by GC­ and LC­MS. Modulation of
inflammation was evaluated with corresponding exhaustive enzymatic hydrolyzates in the model of human neuroblastoma cell line SH­SY5Y. For this, dynamics of proteins involved in inflammation­related signaling was assessed by Luminex xMAP multiparametric immunofluorescence technology. In parallel, profiles of individual AGEs were addressed in the same hydrolyzates by LC­MS/MS. Although no effect on AGE profiles was observed, clear alterations in primary metabolome and regulatory response were observed. This work is supported by the Russian Science Foundation (Grant No 17­16­01042).
Original languageEnglish
Article numberP-28-025
Pages (from-to)286-287
JournalFEBS Open Bio
Volume9
Issue numberS1
DOIs
Publication statusPublished - Jul 2019
Event44th FEBS Congress: From molecules to living systems - Poland, Krakow
Duration: 6 Jul 201911 Jul 2019
Conference number: 44
https://2019.febscongress.org/

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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