Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation

Tatiana V. Lapina, Lidiya Yu Kochemasova, Karl Forchhammer, Elena V. Ermilova

Research output

Abstract

Main conclusion: l-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Abstract: Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that l-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.

Original languageEnglish
JournalPlanta
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

lipid bodies
arginine
Arginine
Growth
Chlorophyta
Nitrogen
acetylglutamate kinase
Proteins
proteins
algae
nitrogen
Ornithine
ornithine
Starvation
Ammonium Compounds
glutamates
droplets
starvation
phosphotransferases (kinases)
Triglycerides

Scopus subject areas

  • Genetics
  • Plant Science

Cite this

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title = "Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation",
abstract = "Main conclusion: l-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Abstract: Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that l-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.",
keywords = "GLB-encoded signaling protein, Nitrogen, Nonphotosynthetic green algae, Oil droplets",
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T1 - Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation

AU - Lapina, Tatiana V.

AU - Kochemasova, Lidiya Yu

AU - Forchhammer, Karl

AU - Ermilova, Elena V.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Main conclusion: l-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Abstract: Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that l-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.

AB - Main conclusion: l-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Abstract: Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that l-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.

KW - GLB-encoded signaling protein

KW - Nitrogen

KW - Nonphotosynthetic green algae

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