Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation

Tatiana V. Lapina, Lidiya Yu. Kochemasova, Karl Forchhammer, Elena V. Ermilova

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Main conclusion l-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that l-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.

Original languageEnglish
Pages (from-to)1379-1385
JournalPlanta
Volume250
Issue number4
DOIs
StatePublished - 1 Oct 2019

Scopus subject areas

  • Genetics
  • Plant Science

Keywords

  • GLB-encoded signaling protein
  • Nitrogen
  • Nonphotosynthetic green algae
  • Oil droplets
  • SIGNAL-TRANSDUCTION PROTEIN
  • KINASE
  • ACETYL-COA CARBOXYLASE
  • ASSIMILATION
  • EXPRESSION

Fingerprint

Dive into the research topics of 'Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation'. Together they form a unique fingerprint.

Cite this