Abstract

A procedure for separation and preconcentration of tetracyclines from human serum samples involving magnetic dispersive micro-solid phase extraction was proposed. The extraction efficiency of different tetracyclines was improved with the use of the surfactant coated Fe3O4 magnetic nanoparticles. Sorption mechanism was presented, and the potential use of magnetic Fe3O4 nanoparticles coated with different surfactants for tetracyclines adsorption was demonstrated for the first time. The procedure involved nanoparticle floating in a liquid sample phase for analyte extraction followed by elution and determination by high performance liquid chromatography with diode array detection. Influence of the main involved parameters was studied, the system was dimensioned accordingly. The analytical curves were linear in the ranges of 0.25–10 mg L−1 for tetracycline and 0.10–10 mg L−1 for oxytetracycline or doxycycline. Limits of detection were estimated (IUPAC, 3 concept) as 0.08 mg L−1 for tetracycline, and 0.03 mg L−1 for oxytetracycline or doxycycline. The proposed procedure proved to be fast (10 min), simple (two stages), inexpensive (10 mg of nanoparticles) and was applied to human serum samples. Unlike previously synthesized nanoparticles for tetracyclines separation, the surfactant-coated Fe3O4 nanoparticles can be easily prepared with widely available and low-cost reagents. Moreover, elution of the analytes was accomplished in absence of organic solvents by an aqueous chelating agent solution.
Original languageEnglish
Article number120861
JournalTalanta
Volume214
Publication statusE-pub ahead of print - 24 Feb 2020

Fingerprint Dive into the research topics of 'Effect of surfactant coating of Fe3O4 nanoparticles on magnetic dispersive micro-solid phase extraction of tetracyclines from human serum'. Together they form a unique fingerprint.

Cite this