Effect of Monocyte-Like THP-1 Cells on the Formation of Vascular Tubes by EA.Hy926s Endothelial Cells in the Presence of Cytokines

T. Yu L’vova, O. I. Stepanova, L. S. Okorokova, V. A. Semenov, E. A. Rebezova, S. A. Sel’kov, D. I. Sokolov

Research output

Abstract

The interaction of endothelial cells with cells of the microenvironment, including monocytes/ macrophages, and extracellular matrix during angiogenesis is controlled by cytokines. The stimulating effect bFGF, IL-8, and VEGF on the formation of capillary-like structures by endothelial cells was demonstrated in both monoculture and in co-culture with THP-1 cells; in the latter case, the effects of bFGF and VEGF were more pronounced. IL-8 reduced branching of vascular tubes in co-culture in comparison with monoculture of endothelial cells. Placental growth factor PlGF had no effect of tube formation by endothelial cells in monoculture, but in co-culture with THP-1 cells this cytokine in high concentrations exhibited proangiogenic activity. TGFb inhibited the formation of vascular tubes by endothelial cells and its antiangiogenic potential was more pronounced in co-culture with THP-1 cells.

Original languageEnglish
Pages (from-to)146-151
Number of pages6
JournalBulletin of Experimental Biology and Medicine
Volume159
Issue number1
DOIs
Publication statusPublished - 1 May 2015
Externally publishedYes

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Endothelial cells
Blood Vessels
Monocytes
Coculture Techniques
Endothelial Cells
Cytokines
Interleukin-8
Cell culture
Vascular Endothelial Growth Factor A
Cellular Microenvironment
Macrophages
Extracellular Matrix
Intercellular Signaling Peptides and Proteins

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

L’vova, T. Yu ; Stepanova, O. I. ; Okorokova, L. S. ; Semenov, V. A. ; Rebezova, E. A. ; Sel’kov, S. A. ; Sokolov, D. I. / Effect of Monocyte-Like THP-1 Cells on the Formation of Vascular Tubes by EA.Hy926s Endothelial Cells in the Presence of Cytokines. In: Bulletin of Experimental Biology and Medicine. 2015 ; Vol. 159, No. 1. pp. 146-151.
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abstract = "The interaction of endothelial cells with cells of the microenvironment, including monocytes/ macrophages, and extracellular matrix during angiogenesis is controlled by cytokines. The stimulating effect bFGF, IL-8, and VEGF on the formation of capillary-like structures by endothelial cells was demonstrated in both monoculture and in co-culture with THP-1 cells; in the latter case, the effects of bFGF and VEGF were more pronounced. IL-8 reduced branching of vascular tubes in co-culture in comparison with monoculture of endothelial cells. Placental growth factor PlGF had no effect of tube formation by endothelial cells in monoculture, but in co-culture with THP-1 cells this cytokine in high concentrations exhibited proangiogenic activity. TGFb inhibited the formation of vascular tubes by endothelial cells and its antiangiogenic potential was more pronounced in co-culture with THP-1 cells.",
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Effect of Monocyte-Like THP-1 Cells on the Formation of Vascular Tubes by EA.Hy926s Endothelial Cells in the Presence of Cytokines. / L’vova, T. Yu; Stepanova, O. I.; Okorokova, L. S.; Semenov, V. A.; Rebezova, E. A.; Sel’kov, S. A.; Sokolov, D. I.

In: Bulletin of Experimental Biology and Medicine, Vol. 159, No. 1, 01.05.2015, p. 146-151.

Research output

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AU - L’vova, T. Yu

AU - Stepanova, O. I.

AU - Okorokova, L. S.

AU - Semenov, V. A.

AU - Rebezova, E. A.

AU - Sel’kov, S. A.

AU - Sokolov, D. I.

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