Effect of Humic Acids on the Metabolism of Chlorella vulgaris in a Model Experiment

M. A. Toropkina, A. G. Ryumin, I. O. Kechaikina, S. N. Chukov

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The effect of humic acids (HAs) on physiological processes (photosynthesis, respiration, and abundance) of green microalga Chlorella vulgaris has been studied, and the relationships between the physiological activity of HAs and their structural parameters have been investigated. It has been found that the optimum range of HA concentrations for the growth of C. vulgaris is 0.01-0.03%. In this range, the highest positive effect on total photosynthesis increment is due to hydrophilic HA preparations from fallow soddypodzolic soil (Albic Retisol) and virgin gray soil (Luvic Greyzemic Phaeozem). The minimum stimulation of respiration is noted for all HA preparations in the region of the maximum photosynthesis stimulation. At concentrations above 0.003%, all HA preparations have a negative effect: the rate of photosynthesis in C. vulgaris cells decreases, and their respiration is strongly enhanced. The abundance of C. vulgaris under the effect of all of the studied preparations under illumination increases more rapidly than in the dark. A high positive coefficient of correlation is found between the hydrophilicity of HAs calculated from C-13 NMR data and the photosynthesis rate in C. vulgaris.

Original languageEnglish
Pages (from-to)1294-1300
Number of pages7
JournalEurasian Soil Science
Volume50
Issue number11
DOIs
StatePublished - Nov 2017

Keywords

  • humic substances
  • physiological activity
  • photosynthesus
  • respiration
  • soil biota
  • 13C-NMR
  • amphiphily of humic acids
  • SOIL ORGANIC-MATTER
  • SUBSTANCES
  • ROOT
  • VERMICOMPOST
  • ARABIDOPSIS
  • EXPRESSION
  • GROWTH

Cite this