Does protein glycation impact on the drought-related changes in metabolism and nutritional properties of mature pea (Pisum sativum L.) seeds?

Tatiana Leonova, Veronika Popova, Alexander Tsarev, Christian Henning, Kristina Antonova, Nadezhda Rogovskaya, Maria Vikhnina, Tim Baldensperger, Alena Soboleva, Ekaterina Dinastia, Mandy Dorn, Olga Shiroglasova, Tatiana Grishina, Gerd U. Balcke, Christian Ihling, Galina Smolikova, Sergei Medvedev, Vladimir A. Zhukov, Vladimir Babakov, Igor A. TikhonovichMarcus A. Glomb, Tatiana Bilova, Andrej Frolov

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Protein glycation is usually referred to as an array of non-enzymatic post-translational modifications formed by reducing sugars and carbonyl products of their degradation. The resulting advanced glycation end products (AGEs) represent a heterogeneous group of covalent adducts, known for their pro-inflammatory effects in mammals, and impacting on pathogenesis of metabolic diseases and ageing. In plants, AGEs are the markers of tissue ageing and response to environmental stressors, the most prominent of which is drought. Although water deficit enhances protein glycation in leaves, its effect on seed glycation profiles is still unknown. Moreover, the effect of drought on biological activities of seed protein in mammalian systems is still unstudied with respect to glycation. Therefore, here we address the effects of a short-term drought on the patterns of seed protein-bound AGEs and accompanying alterations in pro-inflammatory properties of seed protein in the context of seed metabolome dynamics. A short-term drought, simulated as polyethylene glycol-induced osmotic stress and applied at the stage of seed filling, resulted in the dramatic suppression of primary seed metabolism, although the secondary metabolome was minimally affected. This was accompanied with significant suppression of NF-kB activation in human SH-SY5Y neuroblastoma cells after a treatment with protein hydrolyzates, isolated from the mature seeds of drought-treated plants. This effect could not be attributed to formation of known AGEs. Most likely, the prospective anti-inflammatory effect of short-term drought is related to antioxidant effect of unknown secondary metabolite protein adducts, or down-regulation of unknown plant-specific AGEs due to suppression of energy metabolism during seed filling.

Original languageEnglish
Article number567
Number of pages29
JournalInternational Journal of Molecular Sciences
Issue number2
StatePublished - 2 Jan 2020

Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


  • Advanced glycation end products (AGEs)
  • Drought
  • Glycation
  • Metabolomics
  • Osmotic stress
  • Pea (Pisum sativum L.)
  • Pro-inflammatory
  • Seed metabolism
  • Seeds
  • SH-SY5Y human neuroblastoma cells
  • Signaling pathways


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