DNA Binding with Acetate Bis(1,10-phenanthroline)silver(I) Monohydrate in a Solution and Metallization of Formed Structures

Nina Kasyanenko, Zhang Qiushi, Vladimir Bakulev, Mikhail Osolodkov, Petr Sokolov, Viktor Demidov

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The study of DNA interaction with the acetate bis(1,10-phenanthroline)silver(I) monohydrate in a solution is of interest both for understanding the mechanism of biological activity of silver compound and for forming ordered structures (DNA fibrils) that can be used to solve various problems in the field of nanotechnology. The analysis of changing the DNA conformation (secondary structure, persistent length and volume effects) during the interaction by the methods of UV spectroscopy with the analysis of DNA melting, circular dichroism, viscosity, flow birefringence, AFM (atomic force microscopy) and SEM (scanning electron microscopy) was performed. The formation of two types of complexes was observed. At lower concentration of compound in DNA solution, silver atoms form the coordination bonds with a macromolecule, while the released phenanthroline ligands intercalate between DNA bases. When the concentration of the compound increases, the phenanthroline ligands form an ordered "layer" around the helix. The excess of silver compounds in the DNA solution (with more than five silver atoms per base pair), DNA precipitation is observed with the formation of long fibrils. It was shown that the binding of silver to DNA during the formation of complexes provides further metallization of the resulting structures with the aid of reducing agents; phenanthroline ligands influence the result of such metallization.

Original languageEnglish
Article number211
Number of pages20
JournalPolymers
Volume9
Issue number6
DOIs
StatePublished - Jun 2017

Keywords

  • DNA
  • bis(1,10-phenanthroline)silver(I) monohydrate
  • DNA fibrils with silver
  • silver reduction on DNA
  • FLUORESCENT SILVER NANOCLUSTERS
  • ANTICANCER ACTIVITY
  • ANTITUMOR-ACTIVITY
  • CRYSTAL-STRUCTURE
  • NANOPARTICLES
  • COMPLEXES
  • 1,10-PHENANTHROLINE
  • POLYMER
  • PHENANTHROLINE
  • DERIVATIVES

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