Different levels of self-sufficiency of the velocity sedimentation method in the study of linear macromolecules

G.M. Pavlov

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4 Citations (Scopus)

Abstract

The general principles for the interpretation of the sedimentation velocity for linear macromolecules are discussed in combination with the characteristics obtained by other hydrodynamic methods. The several levels of self-sufficiency of the sedimentation velocity method are demonstrated. They include scaling correlation between velocity sedimentation coefficient s0 and concentration coefficient ks=Kskss0bks, application of the sedimentation parameter s for molar mass estimation, qualitative conformation zoning of linear macromolecules using the normalized double logarithmic plots (ksML vs [s]/ML). The last example of self-sufficiency of the method is the study of the concentration dependence of the sedimentation velocity in combination with continuous c(s) distribution using general scaling law model of Sedfit software. This model allows to establish the relationship s0=KsMbs for flexible polymers and perform further gross conformational analysis and analysis of the molar mass distribution. It is shown that
Original languageEnglish
Title of host publicationAnalytical Ultracentrifugation
Subtitle of host publicationInstrumentation, Software and Applications
EditorsSusumu Uchiyama, Fumio Arisaka, Walter F. Stafford, Tom Laue
Place of PublicationTokyo
PublisherSpringer
Pages269-307
ISBN (Electronic)978-4-431-55985-6
ISBN (Print)978-4-431-55983-2
DOIs
Publication statusPublished - 2016

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Pavlov, G. M. (2016). Different levels of self-sufficiency of the velocity sedimentation method in the study of linear macromolecules. In S. Uchiyama, F. Arisaka, W. F. Stafford, & T. Laue (Eds.), Analytical Ultracentrifugation: Instrumentation, Software and Applications (pp. 269-307). [14] Tokyo: Springer. https://doi.org/10.1007/978-4-431-55985-6_14