Development of a receptor-targeted gene delivery system using CXCR4 ligand-conjugated cross-linking peptides

A. Egorova, M. Bogacheva, A. Shubina, V. Baranov, A. Kiselev

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Background: Success in gene therapy greatly depends on the efficiency of nucleic acid delivery. Important features of the carriers for gene delivery should include an enhanced transfection ability, targeting of specific receptors and low toxicity. In the present study, we characterized CXCR4-targeted cross-linking peptides modified with an N-terminal fragment of chemokine stromal cell-derived factor-1α as carriers for gene delivery. Methods: We studied three variants of DNA/carrier complexes with different targeting ligand content. The physicochemical characteristics of the complexes, including their DNA-binding and protective ability, interaction with glycosaminoglycans and size, were determined. Transfection efficacy was studied in cell lines with different levels of CXCR4 expression (HeLa, A172, CHO, Ye{cyrillic}.A{cyrillic}.hy926) and also in human mesenchymal stem cells (hMSCs). The influence of the ligand content on the efficacy of transfection was studied by means of chlorpromazine blockage of clathrin
Original languageUndefined
Pages (from-to)336-351
JournalJournal of Gene Medicine
Volume16
Issue number11-12
StatePublished - 2014

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