Determination of protein adducts of organophosphorus nerve agents in blood plasma

N. L. Koryagina, E. I. Savel’eva, G. V. Karakashev, V. N. Babakov, Ya A. Dubrovskii, E. S. Ukolova, N. S. Khlebnikova, E. A. Murashko, V. Yu Koneva, A. I. Ukolov, V. A. Kopeikin, A. S. Radilov

Research output

5 Citations (Scopus)

Abstract

A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

Original languageEnglish
Pages (from-to)849-859
Number of pages11
JournalJournal of Analytical Chemistry
Volume71
Issue number8
DOIs
Publication statusPublished - 1 Aug 2016

Fingerprint

Blood
Plasmas
Proteins
Spectrometry
Butyrylcholinesterase
Poisons
Biomarkers
Nerve Agents
Serum Albumin
Tyrosine
Blood Proteins
Albumins
Aging of materials
Gases
Liquids
Chemical analysis

Scopus subject areas

  • Analytical Chemistry

Cite this

Koryagina, N. L., Savel’eva, E. I., Karakashev, G. V., Babakov, V. N., Dubrovskii, Y. A., Ukolova, E. S., ... Radilov, A. S. (2016). Determination of protein adducts of organophosphorus nerve agents in blood plasma. Journal of Analytical Chemistry, 71(8), 849-859. https://doi.org/10.1134/S1061934816080086
Koryagina, N. L. ; Savel’eva, E. I. ; Karakashev, G. V. ; Babakov, V. N. ; Dubrovskii, Ya A. ; Ukolova, E. S. ; Khlebnikova, N. S. ; Murashko, E. A. ; Koneva, V. Yu ; Ukolov, A. I. ; Kopeikin, V. A. ; Radilov, A. S. / Determination of protein adducts of organophosphorus nerve agents in blood plasma. In: Journal of Analytical Chemistry. 2016 ; Vol. 71, No. 8. pp. 849-859.
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Koryagina, NL, Savel’eva, EI, Karakashev, GV, Babakov, VN, Dubrovskii, YA, Ukolova, ES, Khlebnikova, NS, Murashko, EA, Koneva, VY, Ukolov, AI, Kopeikin, VA & Radilov, AS 2016, 'Determination of protein adducts of organophosphorus nerve agents in blood plasma', Journal of Analytical Chemistry, vol. 71, no. 8, pp. 849-859. https://doi.org/10.1134/S1061934816080086

Determination of protein adducts of organophosphorus nerve agents in blood plasma. / Koryagina, N. L.; Savel’eva, E. I.; Karakashev, G. V.; Babakov, V. N.; Dubrovskii, Ya A.; Ukolova, E. S.; Khlebnikova, N. S.; Murashko, E. A.; Koneva, V. Yu; Ukolov, A. I.; Kopeikin, V. A.; Radilov, A. S.

In: Journal of Analytical Chemistry, Vol. 71, No. 8, 01.08.2016, p. 849-859.

Research output

TY - JOUR

T1 - Determination of protein adducts of organophosphorus nerve agents in blood plasma

AU - Koryagina, N. L.

AU - Savel’eva, E. I.

AU - Karakashev, G. V.

AU - Babakov, V. N.

AU - Dubrovskii, Ya A.

AU - Ukolova, E. S.

AU - Khlebnikova, N. S.

AU - Murashko, E. A.

AU - Koneva, V. Yu

AU - Ukolov, A. I.

AU - Kopeikin, V. A.

AU - Radilov, A. S.

PY - 2016/8/1

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N2 - A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

AB - A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

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KW - HPLC

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DO - 10.1134/S1061934816080086

M3 - Article

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VL - 71

SP - 849

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JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 8

ER -

Koryagina NL, Savel’eva EI, Karakashev GV, Babakov VN, Dubrovskii YA, Ukolova ES et al. Determination of protein adducts of organophosphorus nerve agents in blood plasma. Journal of Analytical Chemistry. 2016 Aug 1;71(8):849-859. https://doi.org/10.1134/S1061934816080086