Determination of protein adducts of organophosphorus nerve agents in blood plasma

N. L. Koryagina, E. I. Savel’eva, G. V. Karakashev, V. N. Babakov, Ya A. Dubrovskii, E. S. Ukolova, N. S. Khlebnikova, E. A. Murashko, V. Yu Koneva, A. I. Ukolov, V. A. Kopeikin, A. S. Radilov

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

Original languageEnglish
Pages (from-to)849-859
Number of pages11
JournalJournal of Analytical Chemistry
Volume71
Issue number8
DOIs
StatePublished - 1 Aug 2016

Scopus subject areas

  • Analytical Chemistry

Keywords

  • blood plasma
  • gas chromatography
  • HPLC
  • markers of exposure
  • mass-spectrometric detection
  • organophosphorus agents
  • retrospective analysis

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