Coordination to Imidazole Ring Switches On Phosphorescence of Platinum Cyclometalated Complexes: The Route to Selective Labeling of Peptides and Proteins via Histidine Residues

Anastasia I. Solomatina, Pavel S Chelushkin, Dmitrii V. Krupenya, Ivan S. Podkorytov, Tatiana O. Artamonova, Vladimir V. Sizov, Alexei S Melnikov, Vladislav V. Gurzhiy, Elena I. Koshel, Vladislav Shcheslavskiy, Sergey P Tunik

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10 Citations (Scopus)

Abstract

In this study we have shown that substitution of chloride ligand for imidazole (Im) ring in cyclometalated platinum complex, Pt(phpy)(PPh3)Cl (1; phpy = 2-phenylpyridine; PPh3 = triphenylphosphine), which is non-emissive in solution, switches on phosphorescence of the resulting compound. Crystallographic and NMR spectroscopic studies of the substitution product showed that the luminescence ignition is a result of Im coordination to give the [Pt(phpy)(Im)(PPh3)]Cl complex. The other imidazole-containing biomolecules such as histidine and histidine-containing peptides and proteins also trigger luminescence of the substitution products. The complex 1 proved to be highly selective towards the imidazole ring coordination that allows site-specific labelling of peptides and proteins with 1 using the route, which is orthogonal to the common bioconjugation schemes via lysine, aspartic/glutamic acids or cysteine and does not require any preliminary modification of a biomolecule. The utility of this approach was demonst
Original languageEnglish
Pages (from-to)426–437
JournalBioconjugate Chemistry
Volume28
Issue number2
DOIs
Publication statusPublished - 2016

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