Research output: Contribution to journal › Article › peer-review
Contribution of neuraminidase of influenza viruses to the sensitivity to serum inhibitors and reassortment efficiency. / Kiseleva, I. V.; Larionova, N. V.; Bazhenova, E. A.; Fedorova, E. A.; Dubrovina, I. A.; Isakova-Sivak, I. N.; Rudenko, L. G.
In: Molecular Genetics, Microbiology and Virology, Vol. 29, No. 3, 01.07.2014, p. 130-138.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Contribution of neuraminidase of influenza viruses to the sensitivity to serum inhibitors and reassortment efficiency
AU - Kiseleva, I. V.
AU - Larionova, N. V.
AU - Bazhenova, E. A.
AU - Fedorova, E. A.
AU - Dubrovina, I. A.
AU - Isakova-Sivak, I. N.
AU - Rudenko, L. G.
N1 - Funding Information: ACKNOWLEDGMENTS This work was partially supported by the Program for Appropriate Technologies in Health (PATH), Washington, United States. Publisher Copyright: © 2014 Allerton Press, Inc. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/7/1
Y1 - 2014/7/1
N2 - Live attenuated influenza vaccine (LAIV) consists of reassortant viruses with hemagglutinin (HA) and neuraminidase (NA) gene segments inherited from the circulating wild-type (WT) parental viruses and six internal protein-encoding gene segments from cold-adapted attenuated master donor viruses (genome composition 6: 2). In this study, we describe the difficulties in development of LAIV strains depending on the phenotypic peculiarities of the WT viruses used for reassortment. Genomic-composition analysis of 849 reassortants revealed that over 80% of reassortants based on the inhibitor-resistant WT viruses inherited WT NA as compared to 26% of reassortants based on the inhibitor-sensitive WT viruses. In addition, the highest percentage of vaccine genotype reassortants was achieved when WT parental viruses were resistant to nonspecific serum inhibitors. We show that NA may play a role in the influenza virus' sensitivity to a nonspecific serum inhibitors. Replacing the NA of the inhibitor-sensitive WT virus with the NA of the inhibitor-resistant master donor virus significantly decreased the sensitivity of the resulting reassortant virus to nonspecific inhibitors.
AB - Live attenuated influenza vaccine (LAIV) consists of reassortant viruses with hemagglutinin (HA) and neuraminidase (NA) gene segments inherited from the circulating wild-type (WT) parental viruses and six internal protein-encoding gene segments from cold-adapted attenuated master donor viruses (genome composition 6: 2). In this study, we describe the difficulties in development of LAIV strains depending on the phenotypic peculiarities of the WT viruses used for reassortment. Genomic-composition analysis of 849 reassortants revealed that over 80% of reassortants based on the inhibitor-resistant WT viruses inherited WT NA as compared to 26% of reassortants based on the inhibitor-sensitive WT viruses. In addition, the highest percentage of vaccine genotype reassortants was achieved when WT parental viruses were resistant to nonspecific serum inhibitors. We show that NA may play a role in the influenza virus' sensitivity to a nonspecific serum inhibitors. Replacing the NA of the inhibitor-sensitive WT virus with the NA of the inhibitor-resistant master donor virus significantly decreased the sensitivity of the resulting reassortant virus to nonspecific inhibitors.
KW - influenza virus
KW - live attenuated influenza vaccine
KW - neuraminidase
KW - reassortment
KW - serum inhibitors
UR - http://www.scopus.com/inward/record.url?scp=84907507410&partnerID=8YFLogxK
U2 - 10.3103/S0891416814030045
DO - 10.3103/S0891416814030045
M3 - Article
C2 - 25335411
AN - SCOPUS:84908499768
VL - 29
SP - 130
EP - 138
JO - Molecular Genetics, Microbiology and Virology
JF - Molecular Genetics, Microbiology and Virology
SN - 0891-4168
IS - 3
ER -
ID: 75087988