Construction of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5

O. Yu Tumanova, V. N. Kuvshinov, M. Sh Azaev, A. E. Masharsky, N. A. Klimov, A. P. Kozlov, A. A. Ilyichev, L. S. Sandakhchiev

Research output

2 Citations (Scopus)

Abstract

A phage peptide library was screened with virus-neutralizing monoclonal antibodies (MAb) 2F5 which recognize a conserved epitope of HIV-1 gp41. Phages that expose peptides specifically binding with MAb 2F5 were selected by ELISA. Amino acid sequence analysis revealed homology to region 662-671 of HIV-1 HB10 gp160 for most peptides. The major role in recognition was ascribed to Asp-664, Lys-665, and Trp-666. The epitope-mimicking peptides were tested for immunogenicity. Antibodies to gp41 were detected in the serum of immunized rabbits.

Original languageEnglish
Pages (from-to)130-133
Number of pages4
JournalMolecular Biology
Volume35
Issue number1
DOIs
Publication statusPublished - 1 Jan 2001
Externally publishedYes

Fingerprint

Neutralizing Antibodies
HIV-1
Epitopes
Viruses
Bacteriophages
Peptides
Peptide Library
Protein Sequence Analysis
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Rabbits
Antibodies
Serum

Scopus subject areas

  • Biophysics
  • Structural Biology

Cite this

Tumanova, O. Y., Kuvshinov, V. N., Azaev, M. S., Masharsky, A. E., Klimov, N. A., Kozlov, A. P., ... Sandakhchiev, L. S. (2001). Construction of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5. Molecular Biology, 35(1), 130-133. https://doi.org/10.1023/A:1004871305946
Tumanova, O. Yu ; Kuvshinov, V. N. ; Azaev, M. Sh ; Masharsky, A. E. ; Klimov, N. A. ; Kozlov, A. P. ; Ilyichev, A. A. ; Sandakhchiev, L. S. / Construction of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5. In: Molecular Biology. 2001 ; Vol. 35, No. 1. pp. 130-133.
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Tumanova, OY, Kuvshinov, VN, Azaev, MS, Masharsky, AE, Klimov, NA, Kozlov, AP, Ilyichev, AA & Sandakhchiev, LS 2001, 'Construction of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5', Molecular Biology, vol. 35, no. 1, pp. 130-133. https://doi.org/10.1023/A:1004871305946

Construction of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5. / Tumanova, O. Yu; Kuvshinov, V. N.; Azaev, M. Sh; Masharsky, A. E.; Klimov, N. A.; Kozlov, A. P.; Ilyichev, A. A.; Sandakhchiev, L. S.

In: Molecular Biology, Vol. 35, No. 1, 01.01.2001, p. 130-133.

Research output

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AU - Tumanova, O. Yu

AU - Kuvshinov, V. N.

AU - Azaev, M. Sh

AU - Masharsky, A. E.

AU - Klimov, N. A.

AU - Kozlov, A. P.

AU - Ilyichev, A. A.

AU - Sandakhchiev, L. S.

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KW - Immunogenicity

KW - Mimicking peptides

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Tumanova OY, Kuvshinov VN, Azaev MS, Masharsky AE, Klimov NA, Kozlov AP et al. Construction of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5. Molecular Biology. 2001 Jan 1;35(1):130-133. https://doi.org/10.1023/A:1004871305946