Acomys cahirinus is a promising model in neuroscience. Their hippocampus is more developed than that of mice. Given the strong relationship between the hippocampus and exploratory behavior, we conducted a comparative investigation of behavior in acomyses and mice using open field, elevated plus-maze, and light/dark box tests. Compared to mice, acomyses: (1) exhibited lower moving activity in the open field test, characterized by reduced moving time, distance walked, and walking velocity; (2) spent more time in the open arms and less time in the central platform of the elevated plus-maze, while spending similar time in the central part of the open field arena; (3) displayed more rearing behavior in the open field test; (4) perform shorter latencies to enter the light compartment in the light/dark box. Significant differences may be attributed to the different exploratory behavior and non-equal levels of the anxiety of these species.