CHROMOSOME HYDROXYMETHYLATION PATTERNS IN HUMAN ZYGOTES AND CLEAVAGE-STAGE EMBRYOS

O.A. Efimova, A.A. Pendina, A.V. Tikhonov, I.D. Fedorova, O.G. Chiryaeva, E.M. Shilnikova, M.A. Bogdanova, I.Y. Kogan, T.V. Kuznetzova, A.M. Gzgzyan, E.K. Ailamazyan, V.S. Baranov, M.I. Krapivin

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17 Citations (Scopus)

Abstract

We report the sequential changes in 5-hydroxymethylcytosine (5hmC) patterns in the genome of human preimplantation embryos during DNA methylation reprogramming. We have studied chromosome hydroxymethylation and methylation patterns in triploid zygotes and blastomeres of cleavage-stage embryos. Using indirect immunofluorescence, we have analyzed the localization of 5hmC and its co-distribution with 5-methylcytosine (5mC) on the QFH-banded metaphase chromosomes. In zygotes, 5hmC accumulates in both parental chromosome sets, but hydroxymethylation is more intensive in the poorly methylated paternal set. In the maternal set, chromosomes are highly methylated, but contain little 5hmC. Hydroxymethylation is highly region specific in both parental chromosome sets: hydroxymethylated loci correspond to R-bands, but not G-bands, and have well-defined borders, which coincide with the R/G-band boundaries. The centromeric regions and heterochromatin at 1q12, 9q12, 16q11.2, and Yq12 contain little 5mC and no 5hmC. We hypot
Original languageUndefined
Pages (from-to)223-233
JournalReproduction
Volume149
Issue number3
Publication statusPublished - 2015
Externally publishedYes

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