Characteristic Profiles of Biologically Active Substances of Blood Plasma Samples from Patients with Tuberculosis Obtained by HPLC

S. A. Solov’eva, E. A. Bessonova, L. A. Kartsova

Research output: Contribution to journalArticlepeer-review

Abstract

Toxic metabolites (N-acetylisoniazid, pyrazinoic acid, and desacetylrifampicin) of first-line anti-tuberculosis drugs (ethambutol, pyrazinamide, rifampicin, and isoniazid) are identified by liquid chromatography–mass spectrometry, which is important in selecting the required dosages of these drugs for the maximum therapeutic effects and the reduction of side effects. Characteristic chromatographic profiles of blood plasma samples from donors with pulmonary tuberculosis (“pathology”) and a clinically healthy group (“norm”) are obtained and processed by principal component analysis and the k-nearest neighbor method. A perspective of this approach for obtaining of additional diagnostic criteria for pulmonary tuberculosis is shown.
Original languageEnglish
Article number74
Pages (from-to)570-573
JournalJournal of Analytical Chemistry
Volume74
Issue number6
DOIs
StatePublished - 2019

Keywords

  • first-line anti-tuberculosis drugs
  • metabolites
  • HPLC–mass spectrometry
  • characteristic profiles of biologically active compounds
  • chemometrics

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