Cellular responses of congenital immunity in the starfish Asterias rubens

I. V. Kudryavtsev, I. S. D'yachkov, A. A. Kazakov, D. P. Kanaikin, A. D. Kharazova, A. V. Polevshchikov

Research outputpeer-review

3 Citations (Scopus)

Abstract

The work deals with analysis of changes of cellular defense factors in the starfish Asterias rubens in response to injection of human erythrocytes (HE). The number of circulating coelomocytes, dynamics of their production of active oxygen forms, activity of peroxidase, and dynamics of elimination of human hemoglobin from coelomic fluid were estimated before immunization with HE as well as at 6-144 h. The number of coelomocytes was counted in Goryaev chamber, production of active oxygen forms was determined in the test of spontaneous and zymosan-induced reduction of Tetrazolium Nitro Blue, peroxidase activity-in a color enzymatic reaction. Time of human hemoglobin elimination from the coelomic fluid was determined by spectrophotometric method by hemoglobin binding with acetone cyanohydrin with formation of a colored product. It is revealed that injection of human erythrocytes into the starfish Asterias rubens leads to a decrease of the number of coelomocytes in 24-96 h and to an increase of their specific production of active oxygen forms in 96-120 h after the HE injection. In coelomic fluid of Asterias rubens the presence of peroxidase activity is established. The circulation time of human hemoglobin released from erythrocytes in coelomic fluid of these animals does not exceed 24 h. It is suggested that the cellular defense reactions are the major factor of the starfish congenital immunity.

Original languageEnglish
Pages (from-to)134-142
Number of pages9
JournalJournal of Evolutionary Biochemistry and Physiology
Volume41
Issue number2
DOIs
Publication statusPublished - 1 Mar 2005

Fingerprint

Asterias
Starfish
Asterias rubens
Asteroidea
hemoglobin
immunity
Immunity
Hemoglobins
Peroxidase
Reactive Oxygen Species
Fluids
fluid
erythrocytes
Erythrocytes
oxygen
peroxidase
Immunization
Zymosan
immunization
injection

Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Physiology

Cite this

Kudryavtsev, I. V. ; D'yachkov, I. S. ; Kazakov, A. A. ; Kanaikin, D. P. ; Kharazova, A. D. ; Polevshchikov, A. V. / Cellular responses of congenital immunity in the starfish Asterias rubens. In: Journal of Evolutionary Biochemistry and Physiology. 2005 ; Vol. 41, No. 2. pp. 134-142.
@article{edc0e4f823d44514a9964f7eaf0ec9b4,
title = "Cellular responses of congenital immunity in the starfish Asterias rubens",
abstract = "The work deals with analysis of changes of cellular defense factors in the starfish Asterias rubens in response to injection of human erythrocytes (HE). The number of circulating coelomocytes, dynamics of their production of active oxygen forms, activity of peroxidase, and dynamics of elimination of human hemoglobin from coelomic fluid were estimated before immunization with HE as well as at 6-144 h. The number of coelomocytes was counted in Goryaev chamber, production of active oxygen forms was determined in the test of spontaneous and zymosan-induced reduction of Tetrazolium Nitro Blue, peroxidase activity-in a color enzymatic reaction. Time of human hemoglobin elimination from the coelomic fluid was determined by spectrophotometric method by hemoglobin binding with acetone cyanohydrin with formation of a colored product. It is revealed that injection of human erythrocytes into the starfish Asterias rubens leads to a decrease of the number of coelomocytes in 24-96 h and to an increase of their specific production of active oxygen forms in 96-120 h after the HE injection. In coelomic fluid of Asterias rubens the presence of peroxidase activity is established. The circulation time of human hemoglobin released from erythrocytes in coelomic fluid of these animals does not exceed 24 h. It is suggested that the cellular defense reactions are the major factor of the starfish congenital immunity.",
author = "Kudryavtsev, {I. V.} and D'yachkov, {I. S.} and Kazakov, {A. A.} and Kanaikin, {D. P.} and Kharazova, {A. D.} and Polevshchikov, {A. V.}",
year = "2005",
month = "3",
day = "1",
doi = "10.1007/s10893-005-0046-y",
language = "English",
volume = "41",
pages = "134--142",
journal = "Journal of Evolutionary Biochemistry and Physiology",
issn = "0022-0930",
publisher = "Pleiades Publishing",
number = "2",

}

Cellular responses of congenital immunity in the starfish Asterias rubens. / Kudryavtsev, I. V.; D'yachkov, I. S.; Kazakov, A. A.; Kanaikin, D. P.; Kharazova, A. D.; Polevshchikov, A. V.

In: Journal of Evolutionary Biochemistry and Physiology, Vol. 41, No. 2, 01.03.2005, p. 134-142.

Research outputpeer-review

TY - JOUR

T1 - Cellular responses of congenital immunity in the starfish Asterias rubens

AU - Kudryavtsev, I. V.

AU - D'yachkov, I. S.

AU - Kazakov, A. A.

AU - Kanaikin, D. P.

AU - Kharazova, A. D.

AU - Polevshchikov, A. V.

PY - 2005/3/1

Y1 - 2005/3/1

N2 - The work deals with analysis of changes of cellular defense factors in the starfish Asterias rubens in response to injection of human erythrocytes (HE). The number of circulating coelomocytes, dynamics of their production of active oxygen forms, activity of peroxidase, and dynamics of elimination of human hemoglobin from coelomic fluid were estimated before immunization with HE as well as at 6-144 h. The number of coelomocytes was counted in Goryaev chamber, production of active oxygen forms was determined in the test of spontaneous and zymosan-induced reduction of Tetrazolium Nitro Blue, peroxidase activity-in a color enzymatic reaction. Time of human hemoglobin elimination from the coelomic fluid was determined by spectrophotometric method by hemoglobin binding with acetone cyanohydrin with formation of a colored product. It is revealed that injection of human erythrocytes into the starfish Asterias rubens leads to a decrease of the number of coelomocytes in 24-96 h and to an increase of their specific production of active oxygen forms in 96-120 h after the HE injection. In coelomic fluid of Asterias rubens the presence of peroxidase activity is established. The circulation time of human hemoglobin released from erythrocytes in coelomic fluid of these animals does not exceed 24 h. It is suggested that the cellular defense reactions are the major factor of the starfish congenital immunity.

AB - The work deals with analysis of changes of cellular defense factors in the starfish Asterias rubens in response to injection of human erythrocytes (HE). The number of circulating coelomocytes, dynamics of their production of active oxygen forms, activity of peroxidase, and dynamics of elimination of human hemoglobin from coelomic fluid were estimated before immunization with HE as well as at 6-144 h. The number of coelomocytes was counted in Goryaev chamber, production of active oxygen forms was determined in the test of spontaneous and zymosan-induced reduction of Tetrazolium Nitro Blue, peroxidase activity-in a color enzymatic reaction. Time of human hemoglobin elimination from the coelomic fluid was determined by spectrophotometric method by hemoglobin binding with acetone cyanohydrin with formation of a colored product. It is revealed that injection of human erythrocytes into the starfish Asterias rubens leads to a decrease of the number of coelomocytes in 24-96 h and to an increase of their specific production of active oxygen forms in 96-120 h after the HE injection. In coelomic fluid of Asterias rubens the presence of peroxidase activity is established. The circulation time of human hemoglobin released from erythrocytes in coelomic fluid of these animals does not exceed 24 h. It is suggested that the cellular defense reactions are the major factor of the starfish congenital immunity.

UR - http://www.scopus.com/inward/record.url?scp=21344450736&partnerID=8YFLogxK

U2 - 10.1007/s10893-005-0046-y

DO - 10.1007/s10893-005-0046-y

M3 - Article

VL - 41

SP - 134

EP - 142

JO - Journal of Evolutionary Biochemistry and Physiology

JF - Journal of Evolutionary Biochemistry and Physiology

SN - 0022-0930

IS - 2

ER -