TY - JOUR

T1 - Cell Proliferation Indices in Regenerating Alitta virens (Annelida, Errantia)

AU - Shalaeva, Alexandra Y.

AU - Kozin , Vitaly V.

N1 - Shalaeva, A.Y.; Kozin, V.V. Cell Proliferation Indices in Regenerating Alitta virens (Annelida, Errantia). Cells 2023, 12, 1354. https://doi.org/10.3390/cells12101354

PY - 2023/5/10

Y1 - 2023/5/10

N2 - In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. Here we try to elucidate the cellular aspects of regeneration by EdU incorporation in intact and posteriorly amputated annelid Alitta virens using quantitative analysis. We found that the main mechanism of blastema formation in A. virens is local dedifferentiation; mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. The resulting regenerative bud had zones of high proliferative activity and consisted of a heterogeneous population of cells that differed in their anterior–posterior positions and in their cell cycle parameters. The data presented allowed for the quantification of cell proliferation in the context of annelid regeneration for the first time. Regenerative cells showed an unprecedentedly high cycle rate and an exceptionally large growth fraction, making this regeneration model especially valuable for studying coordinated cell cycle entry in vivo in response to injury.

AB - In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. Here we try to elucidate the cellular aspects of regeneration by EdU incorporation in intact and posteriorly amputated annelid Alitta virens using quantitative analysis. We found that the main mechanism of blastema formation in A. virens is local dedifferentiation; mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. The resulting regenerative bud had zones of high proliferative activity and consisted of a heterogeneous population of cells that differed in their anterior–posterior positions and in their cell cycle parameters. The data presented allowed for the quantification of cell proliferation in the context of annelid regeneration for the first time. Regenerative cells showed an unprecedentedly high cycle rate and an exceptionally large growth fraction, making this regeneration model especially valuable for studying coordinated cell cycle entry in vivo in response to injury.

KW - Proliferation

KW - cell kinetics

KW - cumulative labeling

KW - quantitative analysis

KW - blastema

KW - regeneration

KW - invertebrates

KW - annelids

KW - proliferation

UR - https://www.mendeley.com/catalogue/17fc7b66-71fa-3ec0-8a95-a42fa9e52b71/

U2 - 10.3390/cells12101354

DO - 10.3390/cells12101354

M3 - Article

VL - 12

JO - Cells

JF - Cells

SN - 2073-4409

IS - 10

M1 - 1354

ER -