Cell-Cycle Control in Embryonic Stem Cells

Pierre Savatier, Anna Malashicheva

Research outputpeer-review

8 Citations (Scopus)

Abstract

Mouse embryonic stem (ES) cells are the in vitro counterparts of the epiblast cells of the early postimplantation embryo. ES cells are highly pluripotent-they can generate all cell types of the adult organism, which reflects the central role of the epiblast as the founder tissue of the whole embryo in rodents. Mouse ES cells display unusual proliferative properties: their derivation does not rely on any immortalizing agent; they cannot enter a quiescence state; they do not undergo senescence; and they can proliferate without apparent limit. They also can multiply in the absence of serum and are not subject to contact inhibition or anchorage dependence. Human ES cells are also immortal and can form tumors in vivo, suggesting that, besides pluripotency, infinite life span and unrestricted growth are intrinsic features of all ES cells. Some fundamental differences in the expression or regulation of cell-cycle control genes-more specifically, in those regulating the G1-S transition in response to mitogenic signals-are likely to underlie the growth properties of mouse ES cells. In the G1 phase, retinoblastoma (RB) is sequentially phosphorylated by complexes of cyclins and cyclin-dependent kinases (CDKs). The lack of control over the G1-S transition in ES cells-and the gain of this control during differentiation-is likely to reflect a similar phenomenon in the early postimplantation embryo. Epiblast cells are highly proliferating and potentially tumorigenic. The onset of gastrulation is associated with an increase in the proliferation rate of epiblast cells as well as with the start of differentiation within the embryo proper.

Original languageEnglish
Title of host publicationHandbook of Stem Cells
Subtitle of host publicationVolume 1-Embryonic Stem Cells
EditorsRobert Lanza
PublisherElsevier
Chapter5
Pages53-62
Number of pages10
Volume1
ISBN (Electronic)9780080533735
ISBN (Print)9780124366435
DOIs
Publication statusPublished - 14 Sep 2004

Fingerprint

Germ Layers
Embryonic Stem Cells
Cell Cycle Checkpoints
Stem cells
Embryonic Structures
Cells
Contact Inhibition
cdc Genes
Gastrulation
Cyclins
Cyclin-Dependent Kinases
Retinoblastoma
G1 Phase
Growth
Rodentia
Serum
Tumors
Genes
Mouse Embryonic Stem Cells
Neoplasms

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Savatier, P., & Malashicheva, A. (2004). Cell-Cycle Control in Embryonic Stem Cells. In R. Lanza (Ed.), Handbook of Stem Cells: Volume 1-Embryonic Stem Cells (Vol. 1, pp. 53-62). Elsevier. https://doi.org/10.1016/B978-012436643-5/50014-6
Savatier, Pierre ; Malashicheva, Anna. / Cell-Cycle Control in Embryonic Stem Cells. Handbook of Stem Cells: Volume 1-Embryonic Stem Cells. editor / Robert Lanza. Vol. 1 Elsevier, 2004. pp. 53-62
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Savatier, P & Malashicheva, A 2004, Cell-Cycle Control in Embryonic Stem Cells. in R Lanza (ed.), Handbook of Stem Cells: Volume 1-Embryonic Stem Cells. vol. 1, Elsevier, pp. 53-62. https://doi.org/10.1016/B978-012436643-5/50014-6

Cell-Cycle Control in Embryonic Stem Cells. / Savatier, Pierre; Malashicheva, Anna.

Handbook of Stem Cells: Volume 1-Embryonic Stem Cells. ed. / Robert Lanza. Vol. 1 Elsevier, 2004. p. 53-62.

Research outputpeer-review

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Savatier P, Malashicheva A. Cell-Cycle Control in Embryonic Stem Cells. In Lanza R, editor, Handbook of Stem Cells: Volume 1-Embryonic Stem Cells. Vol. 1. Elsevier. 2004. p. 53-62 https://doi.org/10.1016/B978-012436643-5/50014-6