Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase

A. V. Sokolov, V. A. Kostevich, E. Y. Varfolomeeva, D. V. Grigorieva, I. V. Gorudko, S. O. Kozlov, I. V. Kudryavtsev, E. V. Mikhalchik, M. V. Filatov, S. N. Cherenkevich, O. M. Panasenko, J. Arnhold, V. B. Vasilyev

Research outputpeer-review

3 Citations (Scopus)

Abstract

CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by MPO, such as HOCl, HOBr, and HOSCN, on its spectral, enzymatic, and anti-inflammatory properties was studied. We monitored the chemiluminescence of lucigenin and luminol along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by PMA or fMLP. Superoxide dismutase activity of CP and its capacity to reduce the production of oxidants in respiratory burst of neutrophils remained virtually unchanged upon modifications caused by HOCl, HOBr, and HOSCN. Meanwhile, the absorption of type I copper ions at 610 nm became reduced, along with a drop in the ferroxidase and amino oxidase activities of CP. Likewise, its inhibitory effect on the halogenating activity of MPO was diminished. Sera of either healthy donors or patients with Wilson disease were co-incubated with neutrophils from healthy volunteers. In these experiments, we observed an inverse relationship between the content of CP in sera and the rate of H2O2 production by activated neutrophils. In conclusion, CP is likely to play a role of an anti-inflammatory factor tempering the neutrophil respiratory burst in the bloodstream despite the MPO-mediated oxidative modifications.

Original languageEnglish
Pages (from-to)457-467
Number of pages11
JournalBiochemistry and Cell Biology
Volume96
Issue number4
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Ceruloplasmin
Respiratory Burst
Oxidants
Peroxidase
Copper
Neutrophils
Anti-Inflammatory Agents
Scopoletin
Luminol
Chemiluminescence
Acute-Phase Proteins
Tempering
Superoxide Dismutase
Assays
Oxidoreductases
Blood
Fluorescence
Ions
Plasmas
Hepatolenticular Degeneration

Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sokolov, A. V. ; Kostevich, V. A. ; Varfolomeeva, E. Y. ; Grigorieva, D. V. ; Gorudko, I. V. ; Kozlov, S. O. ; Kudryavtsev, I. V. ; Mikhalchik, E. V. ; Filatov, M. V. ; Cherenkevich, S. N. ; Panasenko, O. M. ; Arnhold, J. ; Vasilyev, V. B. / Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase. In: Biochemistry and Cell Biology. 2018 ; Vol. 96, No. 4. pp. 457-467.
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title = "Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase",
abstract = "CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by MPO, such as HOCl, HOBr, and HOSCN, on its spectral, enzymatic, and anti-inflammatory properties was studied. We monitored the chemiluminescence of lucigenin and luminol along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by PMA or fMLP. Superoxide dismutase activity of CP and its capacity to reduce the production of oxidants in respiratory burst of neutrophils remained virtually unchanged upon modifications caused by HOCl, HOBr, and HOSCN. Meanwhile, the absorption of type I copper ions at 610 nm became reduced, along with a drop in the ferroxidase and amino oxidase activities of CP. Likewise, its inhibitory effect on the halogenating activity of MPO was diminished. Sera of either healthy donors or patients with Wilson disease were co-incubated with neutrophils from healthy volunteers. In these experiments, we observed an inverse relationship between the content of CP in sera and the rate of H2O2 production by activated neutrophils. In conclusion, CP is likely to play a role of an anti-inflammatory factor tempering the neutrophil respiratory burst in the bloodstream despite the MPO-mediated oxidative modifications.",
keywords = "Ceruloplasmin, Myeloperoxidase, Neutrophils, Respiratory burst, Superoxide dismutase",
author = "Sokolov, {A. V.} and Kostevich, {V. A.} and Varfolomeeva, {E. Y.} and Grigorieva, {D. V.} and Gorudko, {I. V.} and Kozlov, {S. O.} and Kudryavtsev, {I. V.} and Mikhalchik, {E. V.} and Filatov, {M. V.} and Cherenkevich, {S. N.} and Panasenko, {O. M.} and J. Arnhold and Vasilyev, {V. B.}",
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Sokolov, AV, Kostevich, VA, Varfolomeeva, EY, Grigorieva, DV, Gorudko, IV, Kozlov, SO, Kudryavtsev, IV, Mikhalchik, EV, Filatov, MV, Cherenkevich, SN, Panasenko, OM, Arnhold, J & Vasilyev, VB 2018, 'Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase', Biochemistry and Cell Biology, vol. 96, no. 4, pp. 457-467. https://doi.org/10.1139/bcb-2017-0277

Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase. / Sokolov, A. V.; Kostevich, V. A.; Varfolomeeva, E. Y.; Grigorieva, D. V.; Gorudko, I. V.; Kozlov, S. O.; Kudryavtsev, I. V.; Mikhalchik, E. V.; Filatov, M. V.; Cherenkevich, S. N.; Panasenko, O. M.; Arnhold, J.; Vasilyev, V. B.

In: Biochemistry and Cell Biology, Vol. 96, No. 4, 01.01.2018, p. 457-467.

Research outputpeer-review

TY - JOUR

T1 - Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase

AU - Sokolov, A. V.

AU - Kostevich, V. A.

AU - Varfolomeeva, E. Y.

AU - Grigorieva, D. V.

AU - Gorudko, I. V.

AU - Kozlov, S. O.

AU - Kudryavtsev, I. V.

AU - Mikhalchik, E. V.

AU - Filatov, M. V.

AU - Cherenkevich, S. N.

AU - Panasenko, O. M.

AU - Arnhold, J.

AU - Vasilyev, V. B.

PY - 2018/1/1

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N2 - CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by MPO, such as HOCl, HOBr, and HOSCN, on its spectral, enzymatic, and anti-inflammatory properties was studied. We monitored the chemiluminescence of lucigenin and luminol along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by PMA or fMLP. Superoxide dismutase activity of CP and its capacity to reduce the production of oxidants in respiratory burst of neutrophils remained virtually unchanged upon modifications caused by HOCl, HOBr, and HOSCN. Meanwhile, the absorption of type I copper ions at 610 nm became reduced, along with a drop in the ferroxidase and amino oxidase activities of CP. Likewise, its inhibitory effect on the halogenating activity of MPO was diminished. Sera of either healthy donors or patients with Wilson disease were co-incubated with neutrophils from healthy volunteers. In these experiments, we observed an inverse relationship between the content of CP in sera and the rate of H2O2 production by activated neutrophils. In conclusion, CP is likely to play a role of an anti-inflammatory factor tempering the neutrophil respiratory burst in the bloodstream despite the MPO-mediated oxidative modifications.

AB - CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by MPO, such as HOCl, HOBr, and HOSCN, on its spectral, enzymatic, and anti-inflammatory properties was studied. We monitored the chemiluminescence of lucigenin and luminol along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by PMA or fMLP. Superoxide dismutase activity of CP and its capacity to reduce the production of oxidants in respiratory burst of neutrophils remained virtually unchanged upon modifications caused by HOCl, HOBr, and HOSCN. Meanwhile, the absorption of type I copper ions at 610 nm became reduced, along with a drop in the ferroxidase and amino oxidase activities of CP. Likewise, its inhibitory effect on the halogenating activity of MPO was diminished. Sera of either healthy donors or patients with Wilson disease were co-incubated with neutrophils from healthy volunteers. In these experiments, we observed an inverse relationship between the content of CP in sera and the rate of H2O2 production by activated neutrophils. In conclusion, CP is likely to play a role of an anti-inflammatory factor tempering the neutrophil respiratory burst in the bloodstream despite the MPO-mediated oxidative modifications.

KW - Ceruloplasmin

KW - Myeloperoxidase

KW - Neutrophils

KW - Respiratory burst

KW - Superoxide dismutase

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